Identification of a Spindlin homolog in gibel carp (Carassius auratus gibelio)
文献类型：期刊论文
| 作者 | Wang, XL; Sun, M; Mei, J; Gui, JF |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| 出版日期 | 2005-06-01 |
| 卷号 | 141期号:2页码:159-167 |
| 关键词 | early embryogenesis embryo Carassius auratus gibelio oocyte maturation fertilization beta-tubulin microtubule spindlin (Spin) |
| ISSN号 | 1096-4959 |
| 通讯作者 | Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrol,Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Spindlin has been suggested to play an important role during the transition from oocyte maturation to embryo development in mouse, but its homolog similar to the mouse Spindlin in molecular and expression characterization has not been identified up to now in other vertebrates. In this study, a full length of cDNA sequence is cloned and sequenced from the gibel carp (Carassius auratus gibelio). It contains 1240 nucleotides with an open reading frame of 771 nt encoding 257 amino acids. Based on its amino acid sequence alignment and comparison analysis with the known Spin family proteins, the newly cloned Spin is named Carassius auratus gibelio Spindlin (CagSpin). Its product could be detected from mature eggs to blastula embryos, but its content decreased from the two-cell stage, and could not be detected after the gastrula stage. It suggests that the CagSpin should be a maternal protein that is expressed during oocyte maturation, and plays a crucial role in early cleavage of embryogenesis. CagSpin is the first homolog similar to mouse spindlin identified in fish, and also in other vertebrates. GST pull-down assay reveals the first biochemical evidence for the association of CagSpin and p-tubulin, the microtubule component. Therefore, CagSpin may play important functions by interacting with beta-tubulin and other spindle proteins during oocyte maturation and egg fertilization. (c) 2005 Elsevier Inc. All rights reserved. |
| 英文摘要 | Spindlin has been suggested to play an important role during the transition from oocyte maturation to embryo development in mouse, but its homolog similar to the mouse Spindlin in molecular and expression characterization has not been identified up to now in other vertebrates. In this study, a full length of cDNA sequence is cloned and sequenced from the gibel carp (Carassius auratus gibelio). It contains 1240 nucleotides with an open reading frame of 771 nt encoding 257 amino acids. Based on its amino acid sequence alignment and comparison analysis with the known Spin family proteins, the newly cloned Spin is named Carassius auratus gibelio Spindlin (CagSpin). Its product could be detected from mature eggs to blastula embryos, but its content decreased from the two-cell stage, and could not be detected after the gastrula stage. It suggests that the CagSpin should be a maternal protein that is expressed during oocyte maturation, and plays a crucial role in early cleavage of embryogenesis. CagSpin is the first homolog similar to mouse spindlin identified in fish, and also in other vertebrates. GST pull-down assay reveals the first biochemical evidence for the association of CagSpin and p-tubulin, the microtubule component. Therefore, CagSpin may play important functions by interacting with beta-tubulin and other spindle proteins during oocyte maturation and egg fertilization. (c) 2005 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Zoology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 关键词[WOS] | SILVER CRUCIAN CARP ; MAP KINASE ; MEIOTIC MATURATION ; MIDBLASTULA TRANSITION ; OOCYTE MATURATION ; SIGNAL PEPTIDES ; XENOPUS-EMBRYOS ; CELL-DIVISION ; MOUSE OOCYTES ; EGGS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000230194200004 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9222]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, Inst Hydrol,Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, XL,Sun, M,Mei, J,et al. Identification of a Spindlin homolog in gibel carp (Carassius auratus gibelio)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2005,141(2):159-167. |
| APA | Wang, XL,Sun, M,Mei, J,&Gui, JF.(2005).Identification of a Spindlin homolog in gibel carp (Carassius auratus gibelio).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,141(2),159-167. |
| MLA | Wang, XL,et al."Identification of a Spindlin homolog in gibel carp (Carassius auratus gibelio)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 141.2(2005):159-167. |
入库方式： OAI收割
来源：水生生物研究所
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