Molecular cloning and characterization of the copper/zinc and manganese superoxide dismutase genes from the human parasite Clonorchis sinensis
文献类型：期刊论文
| 作者 | Li, AH; Kong, Y; Cho, SH; Lee, HW; Na, BK; Pak, JK; Kim, TS |
| 刊名 | PARASITOLOGY
 |
| 出版日期 | 2005-06-01 |
| 卷号 | 130页码:687-697 |
| 关键词 | Clonorchis sinensis copper/zinc superoxide dismutase manganese superoxide dismutase cDNA genomic DNA recombinant enzyme helminths |
| ISSN号 | 0031-1820 |
| 通讯作者 | Kim, TS, Natl Inst Hlth, Dept Trop & Endem Parasit Dis, Seoul 122701, South Korea |
| 中文摘要 | A copper/zinc superoxide dismutase (Cu/ZnSOD) gene and a manganese superoxide dismutase (MnSOD) gene of the human parasite Clonorchis sinensis have been cloned and their gene products functionally characterized. Genes Cu/ZnSOD and MnSOD encode proteins of 16 kDa and 25.4 kDa, respectively. The deduced amino acid sequences of the two genes contained highly conserved residues required for activity and secondary structure formation of Cu/ZnSOD and MnSOD, respectively, and show up to 73.7% and 75.4% identities with their counterparts in other animals. The genomic DNA sequence analysis of Cu/ZnSOD gene revealed this as an intronless gene. Inhibitor studies with purified recombinant Cu/ ZnSOD and MnSOD, both of which were functionally expressed in Escherichia coli, confirmed that they are copper/zinc and manganese-containing SOD, respectively. Immunoblots showed that both C. sinensis Cu/ZnSOD and MnSOD should be antigenic for humans, and both, especially the C. sinensis MnSOD, exhibit extensive cross-reactions with sera of patients infected by other trematodes or cestodes. RT-PCR and SOD activity staining of parasite lysates indicate that there are no significant differences in mRNA level or SOD activity for both species of SOD, indicating cytosolic Cu/ZnSOD and MnSOD might play a comparatively important role in the C. sinensis antioxidant system. |
| 英文摘要 | A copper/zinc superoxide dismutase (Cu/ZnSOD) gene and a manganese superoxide dismutase (MnSOD) gene of the human parasite Clonorchis sinensis have been cloned and their gene products functionally characterized. Genes Cu/ZnSOD and MnSOD encode proteins of 16 kDa and 25.4 kDa, respectively. The deduced amino acid sequences of the two genes contained highly conserved residues required for activity and secondary structure formation of Cu/ZnSOD and MnSOD, respectively, and show up to 73.7% and 75.4% identities with their counterparts in other animals. The genomic DNA sequence analysis of Cu/ZnSOD gene revealed this as an intronless gene. Inhibitor studies with purified recombinant Cu/ ZnSOD and MnSOD, both of which were functionally expressed in Escherichia coli, confirmed that they are copper/zinc and manganese-containing SOD, respectively. Immunoblots showed that both C. sinensis Cu/ZnSOD and MnSOD should be antigenic for humans, and both, especially the C. sinensis MnSOD, exhibit extensive cross-reactions with sera of patients infected by other trematodes or cestodes. RT-PCR and SOD activity staining of parasite lysates indicate that there are no significant differences in mRNA level or SOD activity for both species of SOD, indicating cytosolic Cu/ZnSOD and MnSOD might play a comparatively important role in the C. sinensis antioxidant system. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Parasitology |
| 类目[WOS] | Parasitology |
| 研究领域[WOS] | Parasitology |
| 关键词[WOS] | SCHISTOSOMA-MANSONI ; ONCHOCERCA-VOLVULUS ; ESCHERICHIA-COLI ; IDENTIFICATION ; EXPRESSION ; PURIFICATION ; LEISHMANIA ; DAMAGE ; CDNAS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000230011600012 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9226]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Natl Inst Hlth, Dept Trop & Endem Parasit Dis, Seoul 122701, South Korea 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.Sungkyunkwan Univ, Sch Med, Dept Mol Parasitol, Suwon 440746, South Korea 4.Univ Ulsan, Coll Med, Asan Inst Life Sci, Seoul 138736, South Korea |
| 推荐引用方式 GB/T 7714 | Li, AH,Kong, Y,Cho, SH,et al. Molecular cloning and characterization of the copper/zinc and manganese superoxide dismutase genes from the human parasite Clonorchis sinensis[J]. PARASITOLOGY,2005,130:687-697. |
| APA | Li, AH.,Kong, Y.,Cho, SH.,Lee, HW.,Na, BK.,...&Kim, TS.(2005).Molecular cloning and characterization of the copper/zinc and manganese superoxide dismutase genes from the human parasite Clonorchis sinensis.PARASITOLOGY,130,687-697. |
| MLA | Li, AH,et al."Molecular cloning and characterization of the copper/zinc and manganese superoxide dismutase genes from the human parasite Clonorchis sinensis".PARASITOLOGY 130(2005):687-697. |
入库方式： OAI收割
来源：水生生物研究所
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