Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis
文献类型：期刊论文
| 作者 | Zhang, JY; Wu, YS; Wu, HB; Wang, JG; Li, AH; Li, M |
| 刊名 | DISEASES OF AQUATIC ORGANISMS
 |
| 出版日期 | 2005-04-18 |
| 卷号 | 64期号:2页码:121-126 |
| 关键词 | Glugea epinephelusis microsporidia Epinephelus akaara humoral immune responses antigenicity |
| ISSN号 | 0177-5103 |
| 通讯作者 | Wang, JG, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hebei, Peoples R China |
| 中文摘要 | The humoral immune responses of grouper Epinephelus akaara to a natural infection with Glugea epinephelusis was studied by ELISA utilizing intact mature spores as the coated antigen. Results showed that a specific humoral immune response was elicited, but the intensity of infection (in terms of the number of cysts) was not related to the antibody level in naturally infected hosts. The differences in the antigenicity of intact mature spores and soluble spore proteins derived from cracked mature spores were also analyzed. Results suggested that similar antigen epitopes existed between the 2 groups. Additionally, antigen component patterns and the distribution of antigen with immunogenicity were investigated by using the western blot and the immunofluorescent antibody technique (IFAT). The new parasitic microsporidium has specific polypeptide patterns comparable to the reported fish microsporidians. The main antigenic substances are concentrated on the surface of spores, and are mostly located on the anterior and posterior end of the spore bodies. Most surface components of the G. epinephelusis spores are soluble, The potential role of the surface components in initiating infection was also discussed. |
| 英文摘要 | The humoral immune responses of grouper Epinephelus akaara to a natural infection with Glugea epinephelusis was studied by ELISA utilizing intact mature spores as the coated antigen. Results showed that a specific humoral immune response was elicited, but the intensity of infection (in terms of the number of cysts) was not related to the antibody level in naturally infected hosts. The differences in the antigenicity of intact mature spores and soluble spore proteins derived from cracked mature spores were also analyzed. Results suggested that similar antigen epitopes existed between the 2 groups. Additionally, antigen component patterns and the distribution of antigen with immunogenicity were investigated by using the western blot and the immunofluorescent antibody technique (IFAT). The new parasitic microsporidium has specific polypeptide patterns comparable to the reported fish microsporidians. The main antigenic substances are concentrated on the surface of spores, and are mostly located on the anterior and posterior end of the spore bodies. Most surface components of the G. epinephelusis spores are soluble, The potential role of the surface components in initiating infection was also discussed. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Veterinary Sciences |
| 关键词[WOS] | ANGUILLA-JAPONICA TEMMINCK ; HEAD KIDNEY MACROPHAGES ; SCOPHTHALMUS-MAXIMUS L ; PLECOGLOSSUS-ALTIVELIS ; PSEUDOPLEURONECTES-AMERICANUS ; WINTER FLOUNDER ; SPORES ; PROTOZOA ; AYU ; PHAGOCYTOSIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000230065600004 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9228]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hebei, Peoples R China 2.First Mil Med Univ, Inst Trop Med, Guangzhou 510515, Guangdong, Peoples R China 3.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, JY,Wu, YS,Wu, HB,et al. Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis[J]. DISEASES OF AQUATIC ORGANISMS,2005,64(2):121-126. |
| APA | Zhang, JY,Wu, YS,Wu, HB,Wang, JG,Li, AH,&Li, M.(2005).Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis.DISEASES OF AQUATIC ORGANISMS,64(2),121-126. |
| MLA | Zhang, JY,et al."Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis".DISEASES OF AQUATIC ORGANISMS 64.2(2005):121-126. |
入库方式： OAI收割
来源：水生生物研究所
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