Evaluation of soot particles of biomass fuels with endocrine-modulating activity in yeast-based bioassay
文献类型：期刊论文
| 作者 | Wang, JX; Xie, P; Kettrup, A; Schramm, KW |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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| 出版日期 | 2005-04-01 |
| 卷号 | 381期号:8页码:1609-1618 |
| ISSN号 | 1618-2642 |
| 关键词 | biomass fuel soot endocrine modulating disrupter yeast bioassay Reproductive health |
| 通讯作者 | Schramm, KW, GSF NAtl Res Ctr Environm & Hlth, Inst Ecol Chem, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany |
| 中文摘要 | Exposure to indoor air pollution (IAP) from the combustion of biomass fuels is an important cause of morbidity and mortality in developing countries. In the work discussed in this paper we evaluated the endocrine activity of soot particles from biomass fuels by using yeast bioassay. These pollutants could have beta-galactosidase activity with a relative potency (RP) about 10(-7)-10(-9) that of estradiol. Soot particles from wood and straw combustion only partially induced beta-galactosidase activity whereas others produced fully inductive activity in the yeast assay system. These pollutants did not have estrogen antagonist and progesterone agonist activity within the defined concentration range. However, these pollutants require 2-4 orders of magnitude higher IC50 to inhibit the activity of progesterone in a similar dose-response manner to mifepristone. We therefore propose that the endocrine activity of some environmental pollutants may be because of inhibition of the progesterone receptor (hPR). GC-MS results showed that substituted polycyclic aromatic hydrocarbon (PAH) compounds, substituted phenolic compounds and derivatives, aromatic carbonyl compounds, and phytosteroids in these soot particles may be mimicking endogenous hormones. |
| 英文摘要 | Exposure to indoor air pollution (IAP) from the combustion of biomass fuels is an important cause of morbidity and mortality in developing countries. In the work discussed in this paper we evaluated the endocrine activity of soot particles from biomass fuels by using yeast bioassay. These pollutants could have beta-galactosidase activity with a relative potency (RP) about 10(-7)-10(-9) that of estradiol. Soot particles from wood and straw combustion only partially induced beta-galactosidase activity whereas others produced fully inductive activity in the yeast assay system. These pollutants did not have estrogen antagonist and progesterone agonist activity within the defined concentration range. However, these pollutants require 2-4 orders of magnitude higher IC50 to inhibit the activity of progesterone in a similar dose-response manner to mifepristone. We therefore propose that the endocrine activity of some environmental pollutants may be because of inhibition of the progesterone receptor (hPR). GC-MS results showed that substituted polycyclic aromatic hydrocarbon (PAH) compounds, substituted phenolic compounds and derivatives, aromatic carbonyl compounds, and phytosteroids in these soot particles may be mimicking endogenous hormones. |
| 学科主题 | Biochemical Research Methods; Chemistry, Analytical |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | PARTICULATE AIR-POLLUTION ; FINE ORGANIC AEROSOL ; ESTROGEN-RECEPTOR ; ENVIRONMENTAL ESTROGENS ; BREAST-CANCER ; WOOD SMOKE ; COMBUSTION ; CHEMICALS ; ASSAY ; PROGESTERONE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000228728200019 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9280]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.GSF NAtl Res Ctr Environm & Hlth, Inst Ecol Chem, D-85764 Neuherberg, Germany 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China 3.Tech Univ Munich, Lehrstuhl Okol Chem & Umweltanalyt, D-85350 Freising Weihenstephan, Weihenstephan, Germany |
| 推荐引用方式 GB/T 7714 | Wang, JX,Xie, P,Kettrup, A,et al. Evaluation of soot particles of biomass fuels with endocrine-modulating activity in yeast-based bioassay[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2005,381(8):1609-1618. |
| APA | Wang, JX,Xie, P,Kettrup, A,&Schramm, KW.(2005).Evaluation of soot particles of biomass fuels with endocrine-modulating activity in yeast-based bioassay.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,381(8),1609-1618. |
| MLA | Wang, JX,et al."Evaluation of soot particles of biomass fuels with endocrine-modulating activity in yeast-based bioassay".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 381.8(2005):1609-1618. |
入库方式： OAI收割
来源：水生生物研究所
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