Determination of Phenolic Glucosides in Gentiana piasezkii by Capillary Zone Electrophoresis
文献类型：期刊论文
| 作者 | Shi YP(师彦平) ; Yang SR(杨生荣); Shi YP(师彦平)
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| 刊名 | Chromatographia
 |
| 出版日期 | 2005 |
| 卷号 | 62期号:11/12页码:643–647 |
| 关键词 | Capillary electrophoresis Pharmaceutical analysis Phenolic glucosides Gentianaceae Gentiana piasezkii |
| ISSN号 | 0009-5893 |
| 通讯作者 | 师彦平 |
| 中文摘要 | A capillary zone electrophoretic method has been developed for the quantitative analysis of five phenolic glucosides, 6¢-O-vanilloylarbutin (VA), 7-O-feruloylorientin (FE), lutonarin (LN), isoorient (IO) and luteolin (LL), in Gentiana piasezkii with UV detection at 270 nm. 7-O-b-Dglucosyl-coumarin was selected as the internal standard. The applied voltage was 15 kV and the capillary temperature was kept constant at 25℃. The effect of pH, the concentration of methanol and boric acid on migration were studied systematically. Optimum separation was achieved with 200 mM boric acid buffer at pH 9.50 containing 10% (v/v) methanol. Regression equations revealed good linear relationship between the peak area ratio of each compound and internal standard and its concentration. The correlation coefficients were 0.9975, 0.9997, 0.9998, 0.9998 and 0.9988 for VA, FE, LN, IO and LL, respectively. The relative standard deviations of migration time and the peak area ratio of each analyte and internal standard were <1.78% and 4.93%, respectively. The contents of the five compounds in Gentiana piasezkii were successfully determined with satisfactory repeatability and recovery. |
| 学科主题 | 分析化学 ; 药物化学 |
| 收录类别 | SCI |
| 资助信息 | the “Bairen Jihua” Foundation of CAS in 2000;National Natural Science Foundation of China (NSFC No. 20475057;20372029) |
| 语种 | 英语 |
| 公开日期 | 2013-03-01 |
| 源URL | [http://210.77.64.217/handle/362003/2506]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 兰州化学物理研究所_固体润滑国家重点实验室 |
| 通讯作者 | Shi YP(师彦平); Shi YP(师彦平) |
| 推荐引用方式 GB/T 7714 | Shi YP,Yang SR,Shi YP. Determination of Phenolic Glucosides in Gentiana piasezkii by Capillary Zone Electrophoresis[J]. Chromatographia,2005,62(11/12):643–647. |
| APA | 师彦平,杨生荣,&师彦平.(2005).Determination of Phenolic Glucosides in Gentiana piasezkii by Capillary Zone Electrophoresis.Chromatographia,62(11/12),643–647. |
| MLA | 师彦平,et al."Determination of Phenolic Glucosides in Gentiana piasezkii by Capillary Zone Electrophoresis".Chromatographia 62.11/12(2005):643–647. |
入库方式： OAI收割
来源：兰州化学物理研究所
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