Molecular and expression characterization of three gonadotropin subunits common alpha, FSH beta and LH beta in groupers
文献类型：期刊论文
| 作者 | Li, CJ; Zhou, L; Wang, Y; Hong, YH; Gui, JF |
| 刊名 | MOLECULAR AND CELLULAR ENDOCRINOLOGY
 |
| 出版日期 | 2005-04-15 |
| 卷号 | 233期号:1-2页码:33-46 |
| 关键词 | gonadotropins common alpha FSH beta LH beta grouper pituitary |
| ISSN号 | 0303-7207 |
| 通讯作者 | Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | A SMART cDNA plasmid library was constructed from protogyous greasy grouper (Epinephelus coioides) pituitary, and the full-length cDNAs of three gonadotropin (GTH) subunits common alpha, FSH beta and LH beta were cloned and sequenced from the library. The nucleotide sequences of common alpha, FSH beta and LH beta subunit cDNAs are 647, 594 and 574 bp in length, and encode for mature peptides of 94, 99 and 115 aa, respectively. High homology was observed by amino acid sequence alignment and identity comparison of the grouper mature peptides of common alpha, FSH beta and LH beta with that of other fishes. Phylogenetic tree analyses of the three GTH mature subunits revealed similar phylogeny relationships among the studied fish species. Three polyclonal antibodies were prepared from the in vitro expressed common alpha, FSH beta and LH beta mature proteins, respectively. Western blot analysis and immunofluoresence localization were performed on two typical stages of ovarian development stages in red-spotted grouper. Significant differences in protein expression levels of three gonadotropin subunits were revealed between the two ovarian development stages. In the individuals with resting ovary, common alpha was almost not detected in pituitaries, and FSH beta and LH beta expression levels were very low. While in the individuals with developing ovary, the expression of all three gonadotropin subunits reached to a high level. Immunofluoresence localization indicated that the grouper FSH beta cells mainly distributed in the middle area of PPD, while the LH beta cells distributed more widely, including in the area similar to the FSH beta cells and at the external periphery of pituitary near to the PI side. The common alpha might be expressed in both FSH beta and LH beta cells. Double immunofluoresence localization further demonstrated FSH beta and LH beta expression in distinct cells in the PPD area, although the FSH beta and LH beta cells were detected in the identical area of PPD. (c) 2005 Elsevier Ireland Ltd. All rights reserved. |
| 英文摘要 | A SMART cDNA plasmid library was constructed from protogyous greasy grouper (Epinephelus coioides) pituitary, and the full-length cDNAs of three gonadotropin (GTH) subunits common alpha, FSH beta and LH beta were cloned and sequenced from the library. The nucleotide sequences of common alpha, FSH beta and LH beta subunit cDNAs are 647, 594 and 574 bp in length, and encode for mature peptides of 94, 99 and 115 aa, respectively. High homology was observed by amino acid sequence alignment and identity comparison of the grouper mature peptides of common alpha, FSH beta and LH beta with that of other fishes. Phylogenetic tree analyses of the three GTH mature subunits revealed similar phylogeny relationships among the studied fish species. Three polyclonal antibodies were prepared from the in vitro expressed common alpha, FSH beta and LH beta mature proteins, respectively. Western blot analysis and immunofluoresence localization were performed on two typical stages of ovarian development stages in red-spotted grouper. Significant differences in protein expression levels of three gonadotropin subunits were revealed between the two ovarian development stages. In the individuals with resting ovary, common alpha was almost not detected in pituitaries, and FSH beta and LH beta expression levels were very low. While in the individuals with developing ovary, the expression of all three gonadotropin subunits reached to a high level. Immunofluoresence localization indicated that the grouper FSH beta cells mainly distributed in the middle area of PPD, while the LH beta cells distributed more widely, including in the area similar to the FSH beta cells and at the external periphery of pituitary near to the PI side. The common alpha might be expressed in both FSH beta and LH beta cells. Double immunofluoresence localization further demonstrated FSH beta and LH beta expression in distinct cells in the PPD area, although the FSH beta and LH beta cells were detected in the identical area of PPD. (c) 2005 Elsevier Ireland Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Cell Biology; Endocrinology & Metabolism |
| 类目[WOS] | Cell Biology ; Endocrinology & Metabolism |
| 研究领域[WOS] | Cell Biology ; Endocrinology & Metabolism |
| 关键词[WOS] | 2 DISTINCT GONADOTROPINS ; FOLLICLE-STIMULATING-HORMONE ; MESSENGER-RNA LEVELS ; SALMONID PITUITARY GONADOTROPHS ; TROUT ONCORHYNCHUS-MYKISS ; EEL ANGUILLA-JAPONICA ; SEABREAM PAGRUS-MAJOR ; FEMALE RAINBOW-TROUT ; GTH-II ; GENE-EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000228166800004 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9294]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, CJ,Zhou, L,Wang, Y,et al. Molecular and expression characterization of three gonadotropin subunits common alpha, FSH beta and LH beta in groupers[J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY,2005,233(1-2):33-46. |
| APA | Li, CJ,Zhou, L,Wang, Y,Hong, YH,&Gui, JF.(2005).Molecular and expression characterization of three gonadotropin subunits common alpha, FSH beta and LH beta in groupers.MOLECULAR AND CELLULAR ENDOCRINOLOGY,233(1-2),33-46. |
| MLA | Li, CJ,et al."Molecular and expression characterization of three gonadotropin subunits common alpha, FSH beta and LH beta in groupers".MOLECULAR AND CELLULAR ENDOCRINOLOGY 233.1-2(2005):33-46. |
入库方式： OAI收割
来源：水生生物研究所
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