Cultured gill epithelial cells from tilapia (Oreochromis niloticus): a new in vitro assay for toxicants
文献类型：期刊论文
| 作者 | Zhou, BS; Liu, WH; Wu, RSS; Lam, PKS |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2005-01-18 |
| 卷号 | 71期号:1页码:61-72 |
| 关键词 | gill epithelia primary cell culture EROD tilapia B[a]P PCB TCDD |
| ISSN号 | 0166-445X |
| 通讯作者 | Zhou, BS, City Univ Hong Kong, Dept Biol & Chem, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China |
| 中文摘要 | A culture gill epithelium from seawater-adapted tilapia (Oreochromis niloticus) was developed for testing PAHs and dioxin-like contaminants in seawater. The epithelia consists two to three layers of epithelial cells incorporating both pavement cells and mitochondria-rich cells (MRCs). Polarity and a stable transepithelial resistance (TER) were maintained. and closely resembled those in fish gills in vivo. The tightness (integrity) of the epithelia remained unchanged upon exposure to benzo[a]pyrene (B[a]P). 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3,3',4,4',5-pentachlorobiphenyl (PCB#126), while a concentration-dependent response of EROD activity in the epithelia was induced within 18-24 h when the apical side was exposed to these toxicants. The 24 h EC50 of EROD activity was 2.77 x 10(-7) M for PCB#126, 1.85 x 10(-7) M for B[a]P and 7.38 x 10(-10) M for TCDD. showing: that the preparation was not only sensitive to PAHs and dioxin-like compounds, but also able to produce inductive potency of AhR agonists that generally agreed with those derived from other established in vitro and in vivo systems. The results suggest, that the cultured gill epithelia from seawater-adapted tilapia may serve as a simple. rapid and cost-effective tool for assessing exposure and potential effects of toxicants in marine waters. (C) 2004 Elsevier B.V. All rights reserved. |
| 英文摘要 | A culture gill epithelium from seawater-adapted tilapia (Oreochromis niloticus) was developed for testing PAHs and dioxin-like contaminants in seawater. The epithelia consists two to three layers of epithelial cells incorporating both pavement cells and mitochondria-rich cells (MRCs). Polarity and a stable transepithelial resistance (TER) were maintained. and closely resembled those in fish gills in vivo. The tightness (integrity) of the epithelia remained unchanged upon exposure to benzo[a]pyrene (B[a]P). 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3,3',4,4',5-pentachlorobiphenyl (PCB#126), while a concentration-dependent response of EROD activity in the epithelia was induced within 18-24 h when the apical side was exposed to these toxicants. The 24 h EC50 of EROD activity was 2.77 x 10(-7) M for PCB#126, 1.85 x 10(-7) M for B[a]P and 7.38 x 10(-10) M for TCDD. showing: that the preparation was not only sensitive to PAHs and dioxin-like compounds, but also able to produce inductive potency of AhR agonists that generally agreed with those derived from other established in vitro and in vivo systems. The results suggest, that the cultured gill epithelia from seawater-adapted tilapia may serve as a simple. rapid and cost-effective tool for assessing exposure and potential effects of toxicants in marine waters. (C) 2004 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Marine & Freshwater Biology; Toxicology |
| 类目[WOS] | Marine & Freshwater Biology ; Toxicology |
| 研究领域[WOS] | Marine & Freshwater Biology ; Toxicology |
| 关键词[WOS] | MITOCHONDRIA-RICH CELLS ; O-DEETHYLASE INDUCTION ; DIOXIN-LIKE POLLUTANTS ; WATER RAINBOW-TROUT ; FRESH-WATER ; FUNDULUS-HETEROCLITUS ; BRANCHIAL EPITHELIA ; FISH GILL ; POLYCHLORINATED-BIPHENYLS ; AH RECEPTOR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000226566400006 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9336]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.City Univ Hong Kong, Dept Biol & Chem, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, BS,Liu, WH,Wu, RSS,et al. Cultured gill epithelial cells from tilapia (Oreochromis niloticus): a new in vitro assay for toxicants[J]. AQUATIC TOXICOLOGY,2005,71(1):61-72. |
| APA | Zhou, BS,Liu, WH,Wu, RSS,&Lam, PKS.(2005).Cultured gill epithelial cells from tilapia (Oreochromis niloticus): a new in vitro assay for toxicants.AQUATIC TOXICOLOGY,71(1),61-72. |
| MLA | Zhou, BS,et al."Cultured gill epithelial cells from tilapia (Oreochromis niloticus): a new in vitro assay for toxicants".AQUATIC TOXICOLOGY 71.1(2005):61-72. |
入库方式： OAI收割
来源：水生生物研究所
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