中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Enhanced resistance to Aeromonas hydrophila infection and enhanced phagocytic activities in human lactoferrin-transgenic grass carp (Ctenopharyngodon idellus)

文献类型:期刊论文

作者Mao, WF; Wang, YP; Wang, WB; Bo, W; Feng, JX; Zhu, ZY
刊名AQUACULTURE
出版日期2004-12-20
卷号242期号:1-4页码:93-103
关键词sperm-mediated gene transfer lactoferrin Aeromonas hydrophila phagocytosis of kidney cells grass carp Ctenopharyngodon idellus
ISSN号0044-8486
通讯作者Zhu, ZY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要Human lactoferrin (hLF) is an iron-binding protein with antimicrobial and immunomodulatory activities. hLF cDNA was transferred into grass carp via electroporated sperm. The production of transgenic fish was as high as 55% tinder the best parameters. 2(11) pulses and 20-min incubation. The expression of the transgene was demonstrated by the detection of hLF mRNA by RT-PCR. We also investigated the response of G(0) transgenic grass carp to Aeromonas hydrophila infection. Serum lysozyme activities (P>0.05) and phagocytic activities of kidney cells (P<0.05) were measured in transgenic individuals. The transgenic fish not only cleared A. hydrophila significantly faster than the control carp (P<0.05), but also showed enhanced phagocytic activities. The result shows that hLF has immunomodulatory activities in hLF-transgenic grass carp. The transgenic grass carp exhibited enhanced immunity to A. hydrophila infection. These results reveal that the mechanisms of disease resistance are different between hLF-transgenic plants and hLF-transgenic grass carp. (C) 2004 Elsevier B.V. All rights reserved.
英文摘要Human lactoferrin (hLF) is an iron-binding protein with antimicrobial and immunomodulatory activities. hLF cDNA was transferred into grass carp via electroporated sperm. The production of transgenic fish was as high as 55% tinder the best parameters. 2(11) pulses and 20-min incubation. The expression of the transgene was demonstrated by the detection of hLF mRNA by RT-PCR. We also investigated the response of G(0) transgenic grass carp to Aeromonas hydrophila infection. Serum lysozyme activities (P>0.05) and phagocytic activities of kidney cells (P<0.05) were measured in transgenic individuals. The transgenic fish not only cleared A. hydrophila significantly faster than the control carp (P<0.05), but also showed enhanced phagocytic activities. The result shows that hLF has immunomodulatory activities in hLF-transgenic grass carp. The transgenic grass carp exhibited enhanced immunity to A. hydrophila infection. These results reveal that the mechanisms of disease resistance are different between hLF-transgenic plants and hLF-transgenic grass carp. (C) 2004 Elsevier B.V. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科主题Fisheries; Marine & Freshwater Biology
类目[WOS]Fisheries ; Marine & Freshwater Biology
研究领域[WOS]Fisheries ; Marine & Freshwater Biology
关键词[WOS]BOVINE LACTOFERRIN ; CANDIDA-ALBICANS ; POLYMORPHONUCLEAR LEUKOCYTES ; ANTIFUNGAL AGENTS ; IRON ; GENE ; PEPTIDES ; CELLS ; INHIBITION ; GROWTH
收录类别SCI
语种英语
WOS记录号WOS:000225916000007
公开日期2010-10-13
源URL[http://ir.ihb.ac.cn/handle/152342/9352]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文
作者单位Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式
GB/T 7714
Mao, WF,Wang, YP,Wang, WB,et al. Enhanced resistance to Aeromonas hydrophila infection and enhanced phagocytic activities in human lactoferrin-transgenic grass carp (Ctenopharyngodon idellus)[J]. AQUACULTURE,2004,242(1-4):93-103.
APA Mao, WF,Wang, YP,Wang, WB,Bo, W,Feng, JX,&Zhu, ZY.(2004).Enhanced resistance to Aeromonas hydrophila infection and enhanced phagocytic activities in human lactoferrin-transgenic grass carp (Ctenopharyngodon idellus).AQUACULTURE,242(1-4),93-103.
MLA Mao, WF,et al."Enhanced resistance to Aeromonas hydrophila infection and enhanced phagocytic activities in human lactoferrin-transgenic grass carp (Ctenopharyngodon idellus)".AQUACULTURE 242.1-4(2004):93-103.

入库方式: OAI收割

来源:水生生物研究所

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