Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip
文献类型：期刊论文
| 作者 | Wang, SH; Wen, JK; Zhou, YF; Zhang, ZP; Yang, RF; Zhang, JB; Chen, J; Zhang, XE |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2004-11-01 |
| 卷号 | 20期号:4页码:807-813 |
| 关键词 | Bacillus anthracis detection DNA chip multiplex PCR anchored primer PCR alkaline phosphatase |
| ISSN号 | 0956-5663 |
| 通讯作者 | Zhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Dept Analyt Biochem, Wuhan 430071, Peoples R China |
| 中文摘要 | Bacillus anthracis can be identified by detecting virulence factor genes located on two plasmids, pXO1 and pXO2. Combining multiplex PCR with arrayed anchored primer PCR and biotin-avidin alkaline phosphatase indicator system, we developed a qualitative DNA chip method for characterization of B. anthracis, and simultaneous confirmation of the species identity independent of plasmid contents. The assay amplifies pag gene (in pXO1), cap gene (in pXO2) and Ba813 gene (a B. anthracis specific chromosomal marker), and the results were indicated by an easy-to-read profile based on the color reaction of alkaline phosphatase. About 1 pg of specific DNA fragments on the chip wells could be detected after PCR. With the proposed method, the avirulent (pXO1(+)/2(-), pXO1(-)/2(+) and pXO1(-)/2(-)) strains of B. anthracis and distinguished 'anthrax-like' strains from other B. cereus group bacteria were unambiguously identified, while the genera other than Bacillus gave no positive signal. (C) 2004 Elsevier B.V. All rights reserved. |
| 英文摘要 | Bacillus anthracis can be identified by detecting virulence factor genes located on two plasmids, pXO1 and pXO2. Combining multiplex PCR with arrayed anchored primer PCR and biotin-avidin alkaline phosphatase indicator system, we developed a qualitative DNA chip method for characterization of B. anthracis, and simultaneous confirmation of the species identity independent of plasmid contents. The assay amplifies pag gene (in pXO1), cap gene (in pXO2) and Ba813 gene (a B. anthracis specific chromosomal marker), and the results were indicated by an easy-to-read profile based on the color reaction of alkaline phosphatase. About 1 pg of specific DNA fragments on the chip wells could be detected after PCR. With the proposed method, the avirulent (pXO1(+)/2(-), pXO1(-)/2(+) and pXO1(-)/2(-)) strains of B. anthracis and distinguished 'anthrax-like' strains from other B. cereus group bacteria were unambiguously identified, while the genera other than Bacillus gave no positive signal. (C) 2004 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 学科主题 | Biophysics; Biotechnology & Applied Microbiology; Chemistry ; Analytical; Electrochemistry; Nanoscience & Nanotechnology |
| 类目[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 研究领域[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 关键词[WOS] | POLYMERASE-CHAIN-REACTION ; MOLECULAR CHARACTERIZATION ; CHROMOSOMAL DNA ; SAMPLES ; SPORES ; PXO1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000225412600016 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9380]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Dept Analyt Biochem, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 3.Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, SH,Wen, JK,Zhou, YF,et al. Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip[J]. BIOSENSORS & BIOELECTRONICS,2004,20(4):807-813. |
| APA | Wang, SH.,Wen, JK.,Zhou, YF.,Zhang, ZP.,Yang, RF.,...&Zhang, XE.(2004).Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip.BIOSENSORS & BIOELECTRONICS,20(4),807-813. |
| MLA | Wang, SH,et al."Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip".BIOSENSORS & BIOELECTRONICS 20.4(2004):807-813. |
入库方式： OAI收割
来源：水生生物研究所
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