Vitellogenin in rare minnow (Gobiocypris rarus): identification and induction by waterborne diethylstilbestrol
文献类型：期刊论文
| 作者 | Zhong, XP; Xu, Y; Liang, Y; Liao, T; Wang, JW |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
 |
| 出版日期 | 2004-03-01 |
| 卷号 | 137期号:3页码:291-298 |
| 关键词 | diethylstilbestrol environmental estrogens experimental animal hepatosomatic index rare minnow vitellogenin |
| ISSN号 | 1532-0456 |
| 通讯作者 | Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Rare minnow (Gobiocypris rarus) is a tiny Chinese carp that has a short life cycle and is easily cultured in the laboratory. In this study, juvenile rare minnows were exposed to waterborne diethylstilbestrol (DES) at 0.05, 0.5 and 5 mug/l in laboratory aquaria. After exposure for 4, 8, 13 and 21 days, juvenile fish were collected and vitellogenin (Vtg) was measured in whole body homogenates. Native and SDS electrophoresis followed by Western blotting were performed for Vtg identification, and a non-competitive ELISA was developed. In the DES exposure groups (0.5 and 5 mug/l DES), Vtg appeared after 4 days, increased significantly after 8 days and reached a maximum on day 13. Further, a significant increase in the hepatosomatic index (HSI) was found in the 5 mug/l DES exposure group after 21 days. These results indicate that rare minnow provides a good model for assessing endocrine disruption by environmental estrogens. (C) 2004 Elsevier Inc. All rights reserved. |
| 英文摘要 | Rare minnow (Gobiocypris rarus) is a tiny Chinese carp that has a short life cycle and is easily cultured in the laboratory. In this study, juvenile rare minnows were exposed to waterborne diethylstilbestrol (DES) at 0.05, 0.5 and 5 mug/l in laboratory aquaria. After exposure for 4, 8, 13 and 21 days, juvenile fish were collected and vitellogenin (Vtg) was measured in whole body homogenates. Native and SDS electrophoresis followed by Western blotting were performed for Vtg identification, and a non-competitive ELISA was developed. In the DES exposure groups (0.5 and 5 mug/l DES), Vtg appeared after 4 days, increased significantly after 8 days and reached a maximum on day 13. Further, a significant increase in the hepatosomatic index (HSI) was found in the 5 mug/l DES exposure group after 21 days. These results indicate that rare minnow provides a good model for assessing endocrine disruption by environmental estrogens. (C) 2004 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Endocrinology & Metabolism; Toxicology; Zoology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology |
| 关键词[WOS] | MEDAKA ORYZIAS-LATIPES ; PLATICHTHYS-FLESUS ; BIOMARKER ; 17-BETA-ESTRADIOL ; FLOUNDER ; 4-NONYLPHENOL ; PURIFICATION ; PROTEINS ; EXPOSURE ; ASSAY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000224631400008 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9394]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhong, XP,Xu, Y,Liang, Y,et al. Vitellogenin in rare minnow (Gobiocypris rarus): identification and induction by waterborne diethylstilbestrol[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2004,137(3):291-298. |
| APA | Zhong, XP,Xu, Y,Liang, Y,Liao, T,&Wang, JW.(2004).Vitellogenin in rare minnow (Gobiocypris rarus): identification and induction by waterborne diethylstilbestrol.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,137(3),291-298. |
| MLA | Zhong, XP,et al."Vitellogenin in rare minnow (Gobiocypris rarus): identification and induction by waterborne diethylstilbestrol".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 137.3(2004):291-298. |
入库方式： OAI收割
来源：水生生物研究所
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