Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide Ligation assay on DNA chips
文献类型：期刊论文
| 作者 | Jiao-Yu Deng ; Xian-En Zhang ; Hong-Bing Lu ; Qiang Liu ; Zhi-Ping Zhang ; Ya-Feng Zhou ; Wei-Hong Xie and Zhi-Jun Fu |
| 刊名 | JOURNAL OF CLINICAL MICROBIOLOGY
 |
| 出版日期 | 2004 |
| 卷号 | 42期号:10页码:4850-4852 |
| 关键词 | POLYMERASE-CHAIN-REACTION LINE PROBE ASSAY RAPID DETECTION DRUG-RESISTANCE RPOB MUTATIONS GENE STRAINS |
| 通讯作者 | Zhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 中文摘要 | A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberculosis isolates were analyzed, resulting in 87.3% accuracy and 83.6% concordance relative to DNA sequencing. |
| 学科主题 | Microbiology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9400]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 推荐引用方式 GB/T 7714 | Jiao-Yu Deng,Xian-En Zhang,Hong-Bing Lu,et al. Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide Ligation assay on DNA chips[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2004,42(10):4850-4852. |
| APA | Jiao-Yu Deng.,Xian-En Zhang.,Hong-Bing Lu.,Qiang Liu.,Zhi-Ping Zhang.,...&Wei-Hong Xie and Zhi-Jun Fu.(2004).Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide Ligation assay on DNA chips.JOURNAL OF CLINICAL MICROBIOLOGY,42(10),4850-4852. |
| MLA | Jiao-Yu Deng,et al."Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide Ligation assay on DNA chips".JOURNAL OF CLINICAL MICROBIOLOGY 42.10(2004):4850-4852. |
入库方式： OAI收割
来源：水生生物研究所
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