An ELISA-like time-resolved fluorescence immunoassay for microcystin detection
文献类型：期刊论文
| 作者 | Lei, LM; Wu, YS; Gan, NQ; Song, LR |
| 刊名 | CLINICA CHIMICA ACTA
 |
| 出版日期 | 2004-10-01 |
| 卷号 | 348期号:1-2页码:177-180 |
| 关键词 | time-resolved fluorescence immunoassay ELISA microcystin |
| ISSN号 | 0009-8981 |
| 通讯作者 | Song, LR, CAS, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China |
| 中文摘要 | Background: A time-resolved fluorescence immunoassay (TRFIA), based on anti-microcystin-LR (MCLR) monoclonal antibodies (MAbs) and europium-labeled antimouse IgG conjugate, was first developed for microcystin detection. Methods: Anti-MCLR MAbs were prepared by a standard method, and the attained MAbs showed a good cross reactivity with MCLR, MCRR and MCYR. The TRFIA was performed in an indirect competitive mode. The detection method of TRFIA was compared with indirect competitive enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). Results: The TRFIA exhibited a typical sigmoidal response for MCLR at concentrations of 0.005-50 ng/ml, with a wide quantitative range between 0.01 and 10 ng/ml, indicating the broadest detective range and the most sensitive of all the methods for microcystins (MCs) detection. Additionally, the TRFIA maintained good reliability through its quantitative range, as evidenced by low coefficients of variation (1.6-12.2%). The toxin data of algal samples assayed from TRFIA were in the same range as those with ELISA and HPLC, implying that the method was reliable and practical for the detection of MCs. Conclusions: The TRFIA may offer a valuable alternative or a substitute for conventional ELISA for microcystin detection. (C) 2004 Elsevier B.V. All rights reserved. |
| 英文摘要 | Background: A time-resolved fluorescence immunoassay (TRFIA), based on anti-microcystin-LR (MCLR) monoclonal antibodies (MAbs) and europium-labeled antimouse IgG conjugate, was first developed for microcystin detection. Methods: Anti-MCLR MAbs were prepared by a standard method, and the attained MAbs showed a good cross reactivity with MCLR, MCRR and MCYR. The TRFIA was performed in an indirect competitive mode. The detection method of TRFIA was compared with indirect competitive enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). Results: The TRFIA exhibited a typical sigmoidal response for MCLR at concentrations of 0.005-50 ng/ml, with a wide quantitative range between 0.01 and 10 ng/ml, indicating the broadest detective range and the most sensitive of all the methods for microcystins (MCs) detection. Additionally, the TRFIA maintained good reliability through its quantitative range, as evidenced by low coefficients of variation (1.6-12.2%). The toxin data of algal samples assayed from TRFIA were in the same range as those with ELISA and HPLC, implying that the method was reliable and practical for the detection of MCs. Conclusions: The TRFIA may offer a valuable alternative or a substitute for conventional ELISA for microcystin detection. (C) 2004 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Medical Laboratory Technology |
| 类目[WOS] | Medical Laboratory Technology |
| 研究领域[WOS] | Medical Laboratory Technology |
| 关键词[WOS] | CYANOBACTERIAL TOXINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000224337100024 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9406]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.CAS, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lei, LM,Wu, YS,Gan, NQ,et al. An ELISA-like time-resolved fluorescence immunoassay for microcystin detection[J]. CLINICA CHIMICA ACTA,2004,348(1-2):177-180. |
| APA | Lei, LM,Wu, YS,Gan, NQ,&Song, LR.(2004).An ELISA-like time-resolved fluorescence immunoassay for microcystin detection.CLINICA CHIMICA ACTA,348(1-2),177-180. |
| MLA | Lei, LM,et al."An ELISA-like time-resolved fluorescence immunoassay for microcystin detection".CLINICA CHIMICA ACTA 348.1-2(2004):177-180. |
入库方式： OAI收割
来源：水生生物研究所
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