Molecular cloning and characterisation of a fish PKR-like gene from cultured CAB cells induced by UV-inactivated virus
文献类型：期刊论文
| 作者 | Hu, CY; Zhang, YB; Huang, GP; Zhang, QY; Gui, HF |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2004-10-01 |
| 卷号 | 17期号:4页码:353-366 |
| 关键词 | double-stranded-RNA-dependent protein kinase interferon CAB cell grass carp hemorrhage virus expression antiviral-relevant gene immune-relevant gene |
| ISSN号 | 1050-4648 |
| 通讯作者 | Gui, HF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | The double-stranded-RNA-dependent protein kinase (PKR) is an important component in an antiviral defence pathway that is mediated by interferon (IFN) in vertebrates. Previously, some important IFN system genes had been identified from an IFN-producing CAB (crucian carp Carassius auratus blastulae embryonic) cells after treatment with UV-inactivated GCHV (grass carp haemorrhage virus). Here, a fish PKR-like gene, named CaPKR-like, is cloned and sequenced from the same virally infected CAB cells. It has 2192 base pairs in length with a largest open reading frame (ORF) encoding a protein of 513 amino acid residues. BLAST search reveals that the putative CaPKR-like protein is most homologous to human PKR and also has a high-level homology with all members of a family of eIF2alpha kinases. Structurally, CaPKR-like possesses a conserved C-terminal catalytic domain of eIF2alpha kinase family and the most similarity to mammalian PKRs. Within its N-terminus, there are no dsRNA-binding domains conserved in mammalian PKRs instead of two putative Z-DNA binding domains (Zalpha). Like mammalian PKRs, CaPKR-like had a very low level of constitutive expression in normal CAB cells but was up-regulated in response to active GCHV, UV-inactivated GCHV and CAB IFN, implying that the transcriptional activation of CaPKR-like by viral infection is mediated possibly by newly produced CAB IFN, which was further supported by using cycloheximide, a potent inhibitor of protein synthesis. The results together suggested that CaPKR-like was the first identified fish gene most similar to mammalian PKRs. (C) 2004 Elsevier Ltd. All rights reserved. |
| 英文摘要 | The double-stranded-RNA-dependent protein kinase (PKR) is an important component in an antiviral defence pathway that is mediated by interferon (IFN) in vertebrates. Previously, some important IFN system genes had been identified from an IFN-producing CAB (crucian carp Carassius auratus blastulae embryonic) cells after treatment with UV-inactivated GCHV (grass carp haemorrhage virus). Here, a fish PKR-like gene, named CaPKR-like, is cloned and sequenced from the same virally infected CAB cells. It has 2192 base pairs in length with a largest open reading frame (ORF) encoding a protein of 513 amino acid residues. BLAST search reveals that the putative CaPKR-like protein is most homologous to human PKR and also has a high-level homology with all members of a family of eIF2alpha kinases. Structurally, CaPKR-like possesses a conserved C-terminal catalytic domain of eIF2alpha kinase family and the most similarity to mammalian PKRs. Within its N-terminus, there are no dsRNA-binding domains conserved in mammalian PKRs instead of two putative Z-DNA binding domains (Zalpha). Like mammalian PKRs, CaPKR-like had a very low level of constitutive expression in normal CAB cells but was up-regulated in response to active GCHV, UV-inactivated GCHV and CAB IFN, implying that the transcriptional activation of CaPKR-like by viral infection is mediated possibly by newly produced CAB IFN, which was further supported by using cycloheximide, a potent inhibitor of protein synthesis. The results together suggested that CaPKR-like was the first identified fish gene most similar to mammalian PKRs. (C) 2004 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | INDUCED PROTEIN-KINASE ; CARASSIUS-AURATUS L. ; Z-DNA ; EIF-2-ALPHA KINASE ; BINDING DOMAIN ; IN-VIVO ; INTERFERON ; RNA ; IDENTIFICATION ; INITIATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000223586100006 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9440]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Nanchang Univ, Col Life Sci & Food Engn, Nanchang 330047, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, CY,Zhang, YB,Huang, GP,et al. Molecular cloning and characterisation of a fish PKR-like gene from cultured CAB cells induced by UV-inactivated virus[J]. FISH & SHELLFISH IMMUNOLOGY,2004,17(4):353-366. |
| APA | Hu, CY,Zhang, YB,Huang, GP,Zhang, QY,&Gui, HF.(2004).Molecular cloning and characterisation of a fish PKR-like gene from cultured CAB cells induced by UV-inactivated virus.FISH & SHELLFISH IMMUNOLOGY,17(4),353-366. |
| MLA | Hu, CY,et al."Molecular cloning and characterisation of a fish PKR-like gene from cultured CAB cells induced by UV-inactivated virus".FISH & SHELLFISH IMMUNOLOGY 17.4(2004):353-366. |
入库方式： OAI收割
来源：水生生物研究所
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