中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Cloning and sequencing of the growth hormone gene of large yellow croaker and its phylogenetic significance

文献类型:期刊论文

作者Chen, Y; Wang, YP; He, SP; Zhu, ZY
刊名BIOCHEMICAL GENETICS
出版日期2004-10-01
卷号42期号:9-10页码:365-375
关键词Sciaenidae Perciformes GH gene phylogenetics
ISSN号0006-2928
通讯作者Zhu, ZY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要Using conserved primers and the PCR reaction, the growth hormone (GH) gene and the 3'-UTR of the large yellow croaker (Pseudosciaena crocea) were amplified and sequenced. The gene structure was analyzed and compared to the GH genes of 5 other percoid fish downloaded from Genbank. Also the GH gene of the large yellow croaker and the genes from 14 Percoidei and 2 Labroidei species were aligned using Clustal X. A matrix of 564 bp was used to construct the phylogenetic tree using maximum parsimony and neighbor-joining methods. Phylogenetic trees by the two methods are identical in most of the clades with high bootstrap support. The results are also identical to those from morphological data. In general, this analysis does not support the monophyly of the families Centropomidae and Carangidae. But our GH gene tree indicates that the representative species of the families Sparidae and Sciaenidae are a monophyletic group.
英文摘要Using conserved primers and the PCR reaction, the growth hormone (GH) gene and the 3'-UTR of the large yellow croaker (Pseudosciaena crocea) were amplified and sequenced. The gene structure was analyzed and compared to the GH genes of 5 other percoid fish downloaded from Genbank. Also the GH gene of the large yellow croaker and the genes from 14 Percoidei and 2 Labroidei species were aligned using Clustal X. A matrix of 564 bp was used to construct the phylogenetic tree using maximum parsimony and neighbor-joining methods. Phylogenetic trees by the two methods are identical in most of the clades with high bootstrap support. The results are also identical to those from morphological data. In general, this analysis does not support the monophyly of the families Centropomidae and Carangidae. But our GH gene tree indicates that the representative species of the families Sparidae and Sciaenidae are a monophyletic group.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科主题Biochemistry & Molecular Biology; Genetics & Heredity
类目[WOS]Biochemistry & Molecular Biology ; Genetics & Heredity
研究领域[WOS]Biochemistry & Molecular Biology ; Genetics & Heredity
关键词[WOS]CARP CTENOPHARYNGODON-IDELLUS ; ENCODING GENE ; EVOLUTIONARY IMPLICATIONS ; RAINBOW-TROUT ; GH GENES ; PROMOTER ; TELEOST
收录类别SCI
语种英语
WOS记录号WOS:000223623500006
公开日期2010-10-13
源URL[http://ir.ihb.ac.cn/handle/152342/9442]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文
作者单位Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式
GB/T 7714
Chen, Y,Wang, YP,He, SP,et al. Cloning and sequencing of the growth hormone gene of large yellow croaker and its phylogenetic significance[J]. BIOCHEMICAL GENETICS,2004,42(9-10):365-375.
APA Chen, Y,Wang, YP,He, SP,&Zhu, ZY.(2004).Cloning and sequencing of the growth hormone gene of large yellow croaker and its phylogenetic significance.BIOCHEMICAL GENETICS,42(9-10),365-375.
MLA Chen, Y,et al."Cloning and sequencing of the growth hormone gene of large yellow croaker and its phylogenetic significance".BIOCHEMICAL GENETICS 42.9-10(2004):365-375.

入库方式: OAI收割

来源:水生生物研究所

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