Cloning and sequencing of the growth hormone gene of large yellow croaker and its phylogenetic significance
文献类型:期刊论文
作者 | Chen, Y; Wang, YP; He, SP; Zhu, ZY |
刊名 | BIOCHEMICAL GENETICS
![]() |
出版日期 | 2004-10-01 |
卷号 | 42期号:9-10页码:365-375 |
关键词 | Sciaenidae Perciformes GH gene phylogenetics |
ISSN号 | 0006-2928 |
通讯作者 | Zhu, ZY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
中文摘要 | Using conserved primers and the PCR reaction, the growth hormone (GH) gene and the 3'-UTR of the large yellow croaker (Pseudosciaena crocea) were amplified and sequenced. The gene structure was analyzed and compared to the GH genes of 5 other percoid fish downloaded from Genbank. Also the GH gene of the large yellow croaker and the genes from 14 Percoidei and 2 Labroidei species were aligned using Clustal X. A matrix of 564 bp was used to construct the phylogenetic tree using maximum parsimony and neighbor-joining methods. Phylogenetic trees by the two methods are identical in most of the clades with high bootstrap support. The results are also identical to those from morphological data. In general, this analysis does not support the monophyly of the families Centropomidae and Carangidae. But our GH gene tree indicates that the representative species of the families Sparidae and Sciaenidae are a monophyletic group. |
英文摘要 | Using conserved primers and the PCR reaction, the growth hormone (GH) gene and the 3'-UTR of the large yellow croaker (Pseudosciaena crocea) were amplified and sequenced. The gene structure was analyzed and compared to the GH genes of 5 other percoid fish downloaded from Genbank. Also the GH gene of the large yellow croaker and the genes from 14 Percoidei and 2 Labroidei species were aligned using Clustal X. A matrix of 564 bp was used to construct the phylogenetic tree using maximum parsimony and neighbor-joining methods. Phylogenetic trees by the two methods are identical in most of the clades with high bootstrap support. The results are also identical to those from morphological data. In general, this analysis does not support the monophyly of the families Centropomidae and Carangidae. But our GH gene tree indicates that the representative species of the families Sparidae and Sciaenidae are a monophyletic group. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
学科主题 | Biochemistry & Molecular Biology; Genetics & Heredity |
类目[WOS] | Biochemistry & Molecular Biology ; Genetics & Heredity |
研究领域[WOS] | Biochemistry & Molecular Biology ; Genetics & Heredity |
关键词[WOS] | CARP CTENOPHARYNGODON-IDELLUS ; ENCODING GENE ; EVOLUTIONARY IMPLICATIONS ; RAINBOW-TROUT ; GH GENES ; PROMOTER ; TELEOST |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000223623500006 |
公开日期 | 2010-10-13 |
源URL | [http://ir.ihb.ac.cn/handle/152342/9442] ![]() |
专题 | 水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文 |
作者单位 | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Y,Wang, YP,He, SP,et al. Cloning and sequencing of the growth hormone gene of large yellow croaker and its phylogenetic significance[J]. BIOCHEMICAL GENETICS,2004,42(9-10):365-375. |
APA | Chen, Y,Wang, YP,He, SP,&Zhu, ZY.(2004).Cloning and sequencing of the growth hormone gene of large yellow croaker and its phylogenetic significance.BIOCHEMICAL GENETICS,42(9-10),365-375. |
MLA | Chen, Y,et al."Cloning and sequencing of the growth hormone gene of large yellow croaker and its phylogenetic significance".BIOCHEMICAL GENETICS 42.9-10(2004):365-375. |
入库方式: OAI收割
来源:水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。