Dioxin-like components in human breast milk collected from Hong Kong and Guangzhou
文献类型：期刊论文
| 作者 | Lai, KP; Li, W; Xu, Y; Wong, MH; Wong, CKC |
| 刊名 | ENVIRONMENTAL RESEARCH
 |
| 出版日期 | 2004-09-01 |
| 卷号 | 96期号:1页码:88-94 |
| 关键词 | H4IIE rat hepatoma EROD CYP1A1 dioxin TCDD |
| ISSN号 | 0013-9351 |
| 通讯作者 | Wong, CKC, Hong Kong Baptist Univ, Inst Nat Resources & Environm Management, Kowloon, Hong Kong, Peoples R China |
| 中文摘要 | The H4IIE rat hepatoma cell line was employed as a cell model to screen 7-ethoxyresorufin O-deethylase (EROD)-TCDD equivalents (EROD-TEQ) of human breast milk samples collected from Hong Kong and Guangzhou, China. The screening methods employed a 96-well plate spectrofluorometer-EROD assay. For cell-line validation, our results demonstrated a dose-dependent increase in the Ah receptor-mediated response (i.e., CYP1A1 mRNA and EROD) of the cells upon exposure to a number of known Ah receptor agonists, including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,7,8-tetrachlorodibenzothiophene, benzo[a]pyrene, and beta-naphthaflavone. TCDD induced CYP1A1 mRNA and EROD was in a close positive correlation (r = 0.98). For the screening of dioxin-like compounds, breast milk samples collected during lactation weeks 3-5 were used. One hundred (from Hong Kong) and 48 (from Guangzhou) breast milk samples were assayed, of which 65% and 68% of the samples, respectively, showed detectable dioxin-like activities using the H4IIE cell EROD screening method. For sixty-five samples from Hong Kong the mean EROD-TEQ values ranged from 58.1 to 96.5 pg/g of milk fat for those aged 21-36 years while 32 samples from Guangzhou had mean values of 98.8-202.1 pg/g of milk fat. In comparisons of the EROD-TEQ values for different age groups from both cities, there were no significant differences (P < 0.05). However, the mean and median EROD-TEQ values of the Guangzhou population were in general higher than those of the Hong Kong population. The results of the present study indicate that it is feasible to use the H4IIE cell-line as a model for screening dioxin-like compounds in human breast milk. In addition, the method is rapid and cost-effective, particularly for a routine and high-throughput sample screening analysis, compared to the costly and time-intensive chemical analytical techniques. (C) 2003 Elsevier Inc. All rights reserved. |
| 英文摘要 | The H4IIE rat hepatoma cell line was employed as a cell model to screen 7-ethoxyresorufin O-deethylase (EROD)-TCDD equivalents (EROD-TEQ) of human breast milk samples collected from Hong Kong and Guangzhou, China. The screening methods employed a 96-well plate spectrofluorometer-EROD assay. For cell-line validation, our results demonstrated a dose-dependent increase in the Ah receptor-mediated response (i.e., CYP1A1 mRNA and EROD) of the cells upon exposure to a number of known Ah receptor agonists, including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,7,8-tetrachlorodibenzothiophene, benzo[a]pyrene, and beta-naphthaflavone. TCDD induced CYP1A1 mRNA and EROD was in a close positive correlation (r = 0.98). For the screening of dioxin-like compounds, breast milk samples collected during lactation weeks 3-5 were used. One hundred (from Hong Kong) and 48 (from Guangzhou) breast milk samples were assayed, of which 65% and 68% of the samples, respectively, showed detectable dioxin-like activities using the H4IIE cell EROD screening method. For sixty-five samples from Hong Kong the mean EROD-TEQ values ranged from 58.1 to 96.5 pg/g of milk fat for those aged 21-36 years while 32 samples from Guangzhou had mean values of 98.8-202.1 pg/g of milk fat. In comparisons of the EROD-TEQ values for different age groups from both cities, there were no significant differences (P < 0.05). However, the mean and median EROD-TEQ values of the Guangzhou population were in general higher than those of the Hong Kong population. The results of the present study indicate that it is feasible to use the H4IIE cell-line as a model for screening dioxin-like compounds in human breast milk. In addition, the method is rapid and cost-effective, particularly for a routine and high-throughput sample screening analysis, compared to the costly and time-intensive chemical analytical techniques. (C) 2003 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Environmental Sciences; Public ; Environmental & Occupational Health |
| 类目[WOS] | Environmental Sciences ; Public, Environmental & Occupational Health |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health |
| 关键词[WOS] | DIBENZO-P-DIOXINS ; YU-CHENG CHILDREN ; POLYCHLORINATED-BIPHENYLS ; ORGANOCHLORINE PESTICIDES ; 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN TCDD ; EXPOSURE ; EQUIVALENTS ; EXPRESSION ; CHEMICALS ; CYTOKINES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000222983900011 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9454]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Hong Kong Baptist Univ, Inst Nat Resources & Environm Management, Kowloon, Hong Kong, Peoples R China 2.Hong Kong Baptist Univ, Dept Biol, Kowloon, Hong Kong, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lai, KP,Li, W,Xu, Y,et al. Dioxin-like components in human breast milk collected from Hong Kong and Guangzhou[J]. ENVIRONMENTAL RESEARCH,2004,96(1):88-94. |
| APA | Lai, KP,Li, W,Xu, Y,Wong, MH,&Wong, CKC.(2004).Dioxin-like components in human breast milk collected from Hong Kong and Guangzhou.ENVIRONMENTAL RESEARCH,96(1),88-94. |
| MLA | Lai, KP,et al."Dioxin-like components in human breast milk collected from Hong Kong and Guangzhou".ENVIRONMENTAL RESEARCH 96.1(2004):88-94. |
入库方式： OAI收割
来源：水生生物研究所
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