Positive selection on multiple antique allelic lineages of transferrin in the polyploid Carassius auratus
文献类型：期刊论文
| 作者 | Yang, L; Gui, JF |
| 刊名 | MOLECULAR BIOLOGY AND EVOLUTION
 |
| 出版日期 | 2004-07-01 |
| 卷号 | 21期号:7页码:1264-1277 |
| ISSN号 | 0737-4038 |
| 关键词 | transferrin polymorphism positive selection balancing selection allele recombination Carassius auratus |
| 通讯作者 | Gui, JF, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China |
| 中文摘要 | Transferrin polymorphism has been studied in the polyploid Carassius auratus by cloning and sequence analysis of cDNAs from its three subspecies C. auratus gibelio, C. auratus auratus, and C. auratus cuvieri. DNA polymorphism of extremely high extent was shown for the transferrin gene by the 248 segregation sites among coding region sequences of its alleles. The deduced amino acid sequences of the transferrin alleles showed variable theoretical physicochemical parameters, which might constitute molecular basis for their electrophoretic heterogeneity. Positive selection was inferred by the replacement/synonymous ratios larger than 1 in partial allelic lineages which was subsequently confirmed by likelihood simulation under neutral or selection models. Furthermore, the correspondent sites to these selected codons were collectively located at two planes in the crystallographic structure of rabbit transferrin, which suggested that the rapid evolution of C. auratus transferrin might correlate to its adaptation to variable environmental elements such as oxygen pressure. The minimal 26 recombination events were detected among coding sequences of C. auratus transferrin, with partial mosaic sequences and breakpoints identified by identity scanning and information site analyses. Phylogenetic analyses revealed multiple antique allelic lineages of transferrin, which was estimated to diverge fifteen to twenty MYA. All these features strongly suggested the role of balancing selection in long persistence of high transferrin polymorphism in C. auratus. Furthermore, owing to its particular evolutionary backgrounds, the silver crucian carp might possess a distinctive balancing selection mechanism. |
| 英文摘要 | Transferrin polymorphism has been studied in the polyploid Carassius auratus by cloning and sequence analysis of cDNAs from its three subspecies C. auratus gibelio, C. auratus auratus, and C. auratus cuvieri. DNA polymorphism of extremely high extent was shown for the transferrin gene by the 248 segregation sites among coding region sequences of its alleles. The deduced amino acid sequences of the transferrin alleles showed variable theoretical physicochemical parameters, which might constitute molecular basis for their electrophoretic heterogeneity. Positive selection was inferred by the replacement/synonymous ratios larger than 1 in partial allelic lineages which was subsequently confirmed by likelihood simulation under neutral or selection models. Furthermore, the correspondent sites to these selected codons were collectively located at two planes in the crystallographic structure of rabbit transferrin, which suggested that the rapid evolution of C. auratus transferrin might correlate to its adaptation to variable environmental elements such as oxygen pressure. The minimal 26 recombination events were detected among coding sequences of C. auratus transferrin, with partial mosaic sequences and breakpoints identified by identity scanning and information site analyses. Phylogenetic analyses revealed multiple antique allelic lineages of transferrin, which was estimated to diverge fifteen to twenty MYA. All these features strongly suggested the role of balancing selection in long persistence of high transferrin polymorphism in C. auratus. Furthermore, owing to its particular evolutionary backgrounds, the silver crucian carp might possess a distinctive balancing selection mechanism. |
| 学科主题 | Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity |
| 关键词[WOS] | SILVER CRUCIAN CARP ; GIBELIO BLOCH ; SERUM TRANSFERRIN ; RECOMBINATION ; EVOLUTION ; SEQUENCE ; GENES ; POLYMORPHISM ; LIKELIHOOD ; DIVERSITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000222334300011 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9466]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China 2.Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, L,Gui, JF. Positive selection on multiple antique allelic lineages of transferrin in the polyploid Carassius auratus[J]. MOLECULAR BIOLOGY AND EVOLUTION,2004,21(7):1264-1277. |
| APA | Yang, L,&Gui, JF.(2004).Positive selection on multiple antique allelic lineages of transferrin in the polyploid Carassius auratus.MOLECULAR BIOLOGY AND EVOLUTION,21(7),1264-1277. |
| MLA | Yang, L,et al."Positive selection on multiple antique allelic lineages of transferrin in the polyploid Carassius auratus".MOLECULAR BIOLOGY AND EVOLUTION 21.7(2004):1264-1277. |
入库方式： OAI收割
来源：水生生物研究所
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