Genetic heterogeneity analysis and RAPD marker detection among four forms of Atrina pectinata Linnaeus
文献类型：期刊论文
| 作者 | Yu, XY; Mao, Y; Wang, MF; Zhou, L; Gui, JF |
| 刊名 | JOURNAL OF SHELLFISH RESEARCH
 |
| 出版日期 | 2004-04-01 |
| 卷号 | 23期号:1页码:165-171 |
| 关键词 | RAPD inarker genetic polymorphism taxonorny Atrina pectinata |
| ISSN号 | 0730-8000 |
| 通讯作者 | Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | Pen shell (Atrina pectinata Linnaeus) can be distinguished into four forms based on the morphololgic characteristics. Genetic similarity, and heterogeneity were analyzed among the four forms by random amplified polymorphic DNA (RAPD) technique using 24 10-nucleotide-long primers. Of these primers, 22 pruners produced well-identifiable RAPD band patterns. Significant differences in RAPD band patterns were revealed among the four forms. A total of 198 polymorphic fragments were scored from 22 pruners. and they are specific for one form, shared by two or three forms. Several pruners, such as S451, S453 S463 S464, S470. S473 and S474, produced abundant band patterns and provided sufficient information for reliable discrimination of the four forms. The average genetic distances and phylogenetic relationships were calculated and analyzed according to the distinguishable fragments. The data indicate that pen shells of form G and form Y are similar not only among individuals within the same form, but also between individuals from the two forms, and that shells of form T and form S are highly divergent. The constructed phylogenetic free matches the average genetic distances. Three clusters were clearly distinguishable, in which two were corresponding to form S and form T respectively and one included forms G and Y. This Study will be benefit to further studies oil the taxonomy and selective breeding of Pinnid species. It is suggested that the four forms of pen shell should be categorized to at least two species taxonomically. |
| 英文摘要 | Pen shell (Atrina pectinata Linnaeus) can be distinguished into four forms based on the morphololgic characteristics. Genetic similarity, and heterogeneity were analyzed among the four forms by random amplified polymorphic DNA (RAPD) technique using 24 10-nucleotide-long primers. Of these primers, 22 pruners produced well-identifiable RAPD band patterns. Significant differences in RAPD band patterns were revealed among the four forms. A total of 198 polymorphic fragments were scored from 22 pruners. and they are specific for one form, shared by two or three forms. Several pruners, such as S451, S453 S463 S464, S470. S473 and S474, produced abundant band patterns and provided sufficient information for reliable discrimination of the four forms. The average genetic distances and phylogenetic relationships were calculated and analyzed according to the distinguishable fragments. The data indicate that pen shells of form G and form Y are similar not only among individuals within the same form, but also between individuals from the two forms, and that shells of form T and form S are highly divergent. The constructed phylogenetic free matches the average genetic distances. Three clusters were clearly distinguishable, in which two were corresponding to form S and form T respectively and one included forms G and Y. This Study will be benefit to further studies oil the taxonomy and selective breeding of Pinnid species. It is suggested that the four forms of pen shell should be categorized to at least two species taxonomically. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Marine & Freshwater Biology |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology |
| 关键词[WOS] | AURATUS-GIBELIO BLOCH ; SILVER CRUCIAN CARP ; POPULATION-GENETICS ; ARBITRARY PRIMERS ; FAMILY OSTREIDAE ; CRASSOSTREA ; SACCOSTREA ; CLONES ; PCR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000221403900023 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9484]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Zhanjiang Ocean Univ, Coll Fisheries, Zhanjiang 524025, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, XY,Mao, Y,Wang, MF,et al. Genetic heterogeneity analysis and RAPD marker detection among four forms of Atrina pectinata Linnaeus[J]. JOURNAL OF SHELLFISH RESEARCH,2004,23(1):165-171. |
| APA | Yu, XY,Mao, Y,Wang, MF,Zhou, L,&Gui, JF.(2004).Genetic heterogeneity analysis and RAPD marker detection among four forms of Atrina pectinata Linnaeus.JOURNAL OF SHELLFISH RESEARCH,23(1),165-171. |
| MLA | Yu, XY,et al."Genetic heterogeneity analysis and RAPD marker detection among four forms of Atrina pectinata Linnaeus".JOURNAL OF SHELLFISH RESEARCH 23.1(2004):165-171. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。