Residual plastids of bleached mutants of Euglena gracilis and their effects on the expression of nucleus-encoded genes
文献类型：期刊论文
| 作者 | Wang, JX; Shi, ZX; Xu, XD |
| 刊名 | PROGRESS IN NATURAL SCIENCE
 |
| 出版日期 | 2004-03-01 |
| 卷号 | 14期号:3页码:213-217 |
| 关键词 | Euglena gracilis bleached mutant residual plastids tightly stacked membranous structure nucleus-encoded genes regulation |
| ISSN号 | 1002-0071 |
| 通讯作者 | Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | Bleached mutants of Euglena gracilis were obtained by treatment with ofloxacin (Ofl) and streptomycin (Sm) respectively. As shown by electron microscopy, the residual plastids contain prothylakoids in an Ofl mutant, and the highly developed and tightly stacked membranous structure found in cells of two Sm, mutants. Nine genes of the plastid genome were examined with PCR, showing that ribosomal protein genes and most other plastid genes were lost in all but one Sm mutant. Using differential display and RT-PCR, it was shown that chloroplast degeneration could cause changes in transcription of certain nucleus-encoded genes during heterotrophic growth in darkness. |
| 英文摘要 | Bleached mutants of Euglena gracilis were obtained by treatment with ofloxacin (Ofl) and streptomycin (Sm) respectively. As shown by electron microscopy, the residual plastids contain prothylakoids in an Ofl mutant, and the highly developed and tightly stacked membranous structure found in cells of two Sm, mutants. Nine genes of the plastid genome were examined with PCR, showing that ribosomal protein genes and most other plastid genes were lost in all but one Sm mutant. Using differential display and RT-PCR, it was shown that chloroplast degeneration could cause changes in transcription of certain nucleus-encoded genes during heterotrophic growth in darkness. |
| WOS标题词 | Science & Technology ; Technology |
| 学科主题 | Multidisciplinary Sciences |
| 类目[WOS] | Materials Science, Multidisciplinary ; Multidisciplinary Sciences |
| 研究领域[WOS] | Materials Science ; Science & Technology - Other Topics |
| 关键词[WOS] | ASTASIA-LONGA ; CHLOROPLASTS ; DISPLAY ; GENOME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000220878700003 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9506]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, JX,Shi, ZX,Xu, XD. Residual plastids of bleached mutants of Euglena gracilis and their effects on the expression of nucleus-encoded genes[J]. PROGRESS IN NATURAL SCIENCE,2004,14(3):213-217. |
| APA | Wang, JX,Shi, ZX,&Xu, XD.(2004).Residual plastids of bleached mutants of Euglena gracilis and their effects on the expression of nucleus-encoded genes.PROGRESS IN NATURAL SCIENCE,14(3),213-217. |
| MLA | Wang, JX,et al."Residual plastids of bleached mutants of Euglena gracilis and their effects on the expression of nucleus-encoded genes".PROGRESS IN NATURAL SCIENCE 14.3(2004):213-217. |
入库方式： OAI收割
来源：水生生物研究所
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