Oligonucleotide ligation assay-based DNA chip for multiplex detection of single nucleotide polymorphism
文献类型：期刊论文
| 作者 | Yuan Mang ; Ya-Feng Zhou ; Zhi-Ping Zhang ; Jiao-Yu Deng ; Xian-En Zhang ; Hong-Bing Lu, Zhi-Jun Fu ; Qiang Liu |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2004 |
| 卷号 | 19期号:10页码:1277-1283 |
| 关键词 | oligonucleotide ligation assay DNA chip single nucleotide polymorphism detection T4 DNA ligase pentamer enzyme-linked assay M. tuberculosis |
| 通讯作者 | Zhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 中文摘要 | An oligonucleotide ligation assay-based DNA chip has been developed to detect single nucleotide polymorphism. Synthesized nonamers, complementary to the flanking sequences of the mutation sites in target DNA, were immobilized onto glass slides through disulfide bonds on their 5' terminus. Allele-specific pentamers annealed adjacent to the nonamers on the complementary target DNA, containing 5'-phosphate groups and biotin labeled 3'-ends, were mixed with the target DNA in tube. Ligation reactions between nonamers and pentamers were carried out on chips in the presence of T4 DNA ligase. Ligation products were directly visualized on chips through enzyme-linked assay. The effect of G:T mismatch at different positions of pentamers on the ligation were evaluated. The results showed that any mismatch between pentamer and the target DNA could lead to the decrease of ligation, which can be detected easily. The established approach was further used for multiplex detection of mutations in rpoB gene of rifampin-resistant Mycobacterium tuberculosis clinical isolates. (C) 2003 Elsevier B.V. All rights reserved. |
| 学科主题 | Biophysics; Biotechnology & Applied Microbiology; Chemistry ; Analytical; Electrochemistry; Nanoscience & Nanotechnology |
| 收录类别 | SCI |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9520]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yuan Mang,Ya-Feng Zhou,Zhi-Ping Zhang,et al. Oligonucleotide ligation assay-based DNA chip for multiplex detection of single nucleotide polymorphism[J]. BIOSENSORS & BIOELECTRONICS,2004,19(10):1277-1283. |
| APA | Yuan Mang.,Ya-Feng Zhou.,Zhi-Ping Zhang.,Jiao-Yu Deng.,Xian-En Zhang.,...&Qiang Liu.(2004).Oligonucleotide ligation assay-based DNA chip for multiplex detection of single nucleotide polymorphism.BIOSENSORS & BIOELECTRONICS,19(10),1277-1283. |
| MLA | Yuan Mang,et al."Oligonucleotide ligation assay-based DNA chip for multiplex detection of single nucleotide polymorphism".BIOSENSORS & BIOELECTRONICS 19.10(2004):1277-1283. |
入库方式： OAI收割
来源：水生生物研究所
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