Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus
文献类型：期刊论文
| 作者 | Wang, HL; Wu, D; Deng, F; Peng, HY; Chen, XW; Lauzon, H; Arif, BM; Jehle, JA; Hu, ZH |
| 刊名 | VIRUS RESEARCH
 |
| 出版日期 | 2004-03-15 |
| 卷号 | 100期号:2页码:179-189 |
| ISSN号 | 0168-1702 |
| 关键词 | HaSNPV chitinase characterization phylogenetic analysis secondary structure analysis |
| 通讯作者 | Hu, ZH, Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China |
| 中文摘要 | A putative chitinase gene was identified within the fragment EcoRI-K of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearNPV, also called HaSNPIV) genome. The open reading frame (ORF) contains 1713 nucleotides (nt) and encodes a protein of 570 amino acids (aa) with a predicted molecular weight of 63.6 kDa. Transcription started at about 18 h post infection (p.i.) and the protein was first detected at 20 h p.i. The times of transcription and expression are characteristic of a late baculovirus gene. 5' and 3' RACE indicated that transcription was initiated from the adenine residue located at -246 nt upstream from the ATG start site and the poly (A) tail was added at 267 nt downstream from the stop codon. This is the first report on the molecular characterization of a chitinase from a single nucleocapsid NPV. The phylogeny of baculoviral chitinase genes were extensively examined in comparison with chitinases derived from bacteria, fungi, nematode, actinomycetes, viruses, insects and mammals. Neighbor-joining and most parsimony analyses showed that the baculoviral chitinases were clustered exclusively within gamma-proteobacteria. Our results strongly suggest that baculoviruses acquired their chitinase genes from bacteria. (C) 2004 Elsevier B.V. All rights reserved. |
| 英文摘要 | A putative chitinase gene was identified within the fragment EcoRI-K of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearNPV, also called HaSNPIV) genome. The open reading frame (ORF) contains 1713 nucleotides (nt) and encodes a protein of 570 amino acids (aa) with a predicted molecular weight of 63.6 kDa. Transcription started at about 18 h post infection (p.i.) and the protein was first detected at 20 h p.i. The times of transcription and expression are characteristic of a late baculovirus gene. 5' and 3' RACE indicated that transcription was initiated from the adenine residue located at -246 nt upstream from the ATG start site and the poly (A) tail was added at 267 nt downstream from the stop codon. This is the first report on the molecular characterization of a chitinase from a single nucleocapsid NPV. The phylogeny of baculoviral chitinase genes were extensively examined in comparison with chitinases derived from bacteria, fungi, nematode, actinomycetes, viruses, insects and mammals. Neighbor-joining and most parsimony analyses showed that the baculoviral chitinases were clustered exclusively within gamma-proteobacteria. Our results strongly suggest that baculoviruses acquired their chitinase genes from bacteria. (C) 2004 Elsevier B.V. All rights reserved. |
| 学科主题 | Virology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Virology |
| 研究领域[WOS] | Virology |
| 关键词[WOS] | NUCLEAR POLYHEDROSIS-VIRUS ; PROTEIN SECONDARY STRUCTURE ; CARBOXY-TERMINAL KDEL ; ENDOPLASMIC-RETICULUM ; SEQUENCE-ANALYSIS ; BACULOVIRUS ; GENOME ; IDENTIFICATION ; RESOLUTION ; ENCODES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000220326400004 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9536]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Mol Virol, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430071, Peoples R China 4.Great Lakes Forestry Res Ctr, Mol Virol Lab, Sault Ste Marie, ON P6A 5M7, Canada 5.State Educ & Res Ctr Agr Viticulture & Hort, Sect Biotechnol Crop Protect, D-67435 Neustadt, Germany |
| 推荐引用方式 GB/T 7714 | Wang, HL,Wu, D,Deng, F,et al. Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus[J]. VIRUS RESEARCH,2004,100(2):179-189. |
| APA | Wang, HL.,Wu, D.,Deng, F.,Peng, HY.,Chen, XW.,...&Hu, ZH.(2004).Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus.VIRUS RESEARCH,100(2),179-189. |
| MLA | Wang, HL,et al."Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus".VIRUS RESEARCH 100.2(2004):179-189. |
入库方式： OAI收割
来源：水生生物研究所
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