A C-type lectin associated and translocated with cortical granules during oocyte maturation and egg fertilization in fish
文献类型：期刊论文
| 作者 | Dong, CH; Yang, ST; Yang, ZA; Zhang, L; Gui, JF |
| 刊名 | DEVELOPMENTAL BIOLOGY
 |
| 出版日期 | 2004-01-15 |
| 卷号 | 265期号:2页码:341-354 |
| 关键词 | lectin cortical granule translocation phosphorylation gibel carp |
| ISSN号 | 0012-1606 |
| 通讯作者 | Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | Oocyte maturation and egg fertilization in both vertebrates and invertebrates are marked by orchestrated cytoplasmic translocation of secretory vesicles known as cortical granules. It is thought that such redistribution of cellular content is critical for asymmetrical cell division during early development, but the mechanism and regulation of the process is poorly understood. Here we report the identification, purification and cDNA cloning of a C-type lectin from oocytes of a freshwater fish species gibel carp (Carassius auratus gibelio). The purified protein has been demonstrated to have lectin activity and to be a Ca2+-dependent C-type lectin by hemagglutination activity assay. Immunocytochemistry revealed that the lectin is associated with cortical granules, gradually translocated to the cell surface during oocyte maturation, and discharged to the egg envelope upon fertilization. Interestingly, the lectin becomes phosphorylated on threonine residues upon induction of exocytosis by fertilization and returns to its original state after morula stage of embryonic development, suggesting that this posttranslational modification may represent a critical molecular switch for early embryonic development. (C) 2003 Elsevier Inc. All rights reserved. |
| 英文摘要 | Oocyte maturation and egg fertilization in both vertebrates and invertebrates are marked by orchestrated cytoplasmic translocation of secretory vesicles known as cortical granules. It is thought that such redistribution of cellular content is critical for asymmetrical cell division during early development, but the mechanism and regulation of the process is poorly understood. Here we report the identification, purification and cDNA cloning of a C-type lectin from oocytes of a freshwater fish species gibel carp (Carassius auratus gibelio). The purified protein has been demonstrated to have lectin activity and to be a Ca2+-dependent C-type lectin by hemagglutination activity assay. Immunocytochemistry revealed that the lectin is associated with cortical granules, gradually translocated to the cell surface during oocyte maturation, and discharged to the egg envelope upon fertilization. Interestingly, the lectin becomes phosphorylated on threonine residues upon induction of exocytosis by fertilization and returns to its original state after morula stage of embryonic development, suggesting that this posttranslational modification may represent a critical molecular switch for early embryonic development. (C) 2003 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Developmental Biology |
| 类目[WOS] | Developmental Biology |
| 研究领域[WOS] | Developmental Biology |
| 关键词[WOS] | SILVER CRUCIAN CARP ; AURATUS-GIBELIO BLOCH ; XENOPUS-LAEVIS ; CAPILLARY-ELECTROPHORESIS ; SPLICING FACTORS ; PROTEIN ; EMBRYOGENESIS ; OOGENESIS ; KINASE ; CLONES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000188931000006 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9554]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, CH,Yang, ST,Yang, ZA,et al. A C-type lectin associated and translocated with cortical granules during oocyte maturation and egg fertilization in fish[J]. DEVELOPMENTAL BIOLOGY,2004,265(2):341-354. |
| APA | Dong, CH,Yang, ST,Yang, ZA,Zhang, L,&Gui, JF.(2004).A C-type lectin associated and translocated with cortical granules during oocyte maturation and egg fertilization in fish.DEVELOPMENTAL BIOLOGY,265(2),341-354. |
| MLA | Dong, CH,et al."A C-type lectin associated and translocated with cortical granules during oocyte maturation and egg fertilization in fish".DEVELOPMENTAL BIOLOGY 265.2(2004):341-354. |
入库方式： OAI收割
来源：水生生物研究所
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