Broad host range plasmid-based gene transfer system in the cyanobacterium Gloeobacter violaceus which lacks thylakoids
文献类型：期刊论文
| 作者 | Guo, HT; Xu, XD |
| 刊名 | PROGRESS IN NATURAL SCIENCE
 |
| 出版日期 | 2004 |
| 卷号 | 14期号:1页码:31-35 |
| 关键词 | Gloeobacter violaceus conjugation pKT210 |
| ISSN号 | 1002-0071 |
| 通讯作者 | Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | Gloeobacter violaceus, a cyanobacterium lack of thylakoids, is refractory to genetic manipulations because its cells are enveloped by a thick gelatinous sheath and in colonial form. In this study, a large number of single cells were obtained by repeated pumping with a syringe with the gelatinous sheath removed. And an exogenous broad host range plasmid pKT210 was conjugatively transferred into G. violaceus. Analyses with dot-blot hybridization and restriction mapping showed that the exogenous plasmid pKT210 had been introduced into G. violaceus and stably maintained with no alteration in its structure. pKT210 extracted from G. violaceus exconjugants could be transformed into the mcr - mrr - E. coli strain DH10B but not the mcr(+) mrr(+) strain DH5alpha, which suggests that a methylase system may be present in G. violaceus. |
| 英文摘要 | Gloeobacter violaceus, a cyanobacterium lack of thylakoids, is refractory to genetic manipulations because its cells are enveloped by a thick gelatinous sheath and in colonial form. In this study, a large number of single cells were obtained by repeated pumping with a syringe with the gelatinous sheath removed. And an exogenous broad host range plasmid pKT210 was conjugatively transferred into G. violaceus. Analyses with dot-blot hybridization and restriction mapping showed that the exogenous plasmid pKT210 had been introduced into G. violaceus and stably maintained with no alteration in its structure. pKT210 extracted from G. violaceus exconjugants could be transformed into the mcr - mrr - E. coli strain DH10B but not the mcr(+) mrr(+) strain DH5alpha, which suggests that a methylase system may be present in G. violaceus. |
| WOS标题词 | Science & Technology ; Technology |
| 学科主题 | Multidisciplinary Sciences |
| 类目[WOS] | Materials Science, Multidisciplinary ; Multidisciplinary Sciences |
| 研究领域[WOS] | Materials Science ; Science & Technology - Other Topics |
| 关键词[WOS] | ESCHERICHIA-COLI ; CONJUGATIVE TRANSFER ; TRANSFORMATION ; VECTORS ; DNA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000188789300006 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9560]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, HT,Xu, XD. Broad host range plasmid-based gene transfer system in the cyanobacterium Gloeobacter violaceus which lacks thylakoids[J]. PROGRESS IN NATURAL SCIENCE,2004,14(1):31-35. |
| APA | Guo, HT,&Xu, XD.(2004).Broad host range plasmid-based gene transfer system in the cyanobacterium Gloeobacter violaceus which lacks thylakoids.PROGRESS IN NATURAL SCIENCE,14(1),31-35. |
| MLA | Guo, HT,et al."Broad host range plasmid-based gene transfer system in the cyanobacterium Gloeobacter violaceus which lacks thylakoids".PROGRESS IN NATURAL SCIENCE 14.1(2004):31-35. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。