alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120
文献类型:期刊论文
作者 | Ning, DG; Xu, XD |
刊名 | MICROBIOLOGY-SGM |
出版日期 | 2004-02-01 |
卷号 | 150期号:0页码:447-453 |
ISSN号 | 1350-0872 |
关键词 | CalA ftsZ DNA-binding protein Anabaena |
通讯作者 | Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
中文摘要 | Anabaena sp. PCC; 7120 was mutagenized by transposon Tn5-1087b, generating a mutant whose heterocysts lack the envelope polysaccharide layer. The transposon was located between nucleotides 342 and 343 of alr0117, a 918 bp gene encoding a histidine kinase for a two-component regulatory system. Complementation of the mutant with a DNA fragment containing alr0117 and targeted inactivation of the gene confirmed that alr0117 is involved in heterocyst development. RT-PCR showed that alr0117 was constitutively expressed in the presence or absence of a combined-nitrogen source. hepA and patB, the two genes turned on during wild-type heterocyst development, were no longer activated in an alr0117-null mutant. The two-component signal transduction system involving alr0117 may control the formation of the envelope polysaccharide layer and certain late events essential to the function of heterocysts. |
英文摘要 | Anabaena sp. PCC; 7120 was mutagenized by transposon Tn5-1087b, generating a mutant whose heterocysts lack the envelope polysaccharide layer. The transposon was located between nucleotides 342 and 343 of alr0117, a 918 bp gene encoding a histidine kinase for a two-component regulatory system. Complementation of the mutant with a DNA fragment containing alr0117 and targeted inactivation of the gene confirmed that alr0117 is involved in heterocyst development. RT-PCR showed that alr0117 was constitutively expressed in the presence or absence of a combined-nitrogen source. hepA and patB, the two genes turned on during wild-type heterocyst development, were no longer activated in an alr0117-null mutant. The two-component signal transduction system involving alr0117 may control the formation of the envelope polysaccharide layer and certain late events essential to the function of heterocysts. |
学科主题 | Microbiology |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Microbiology |
研究领域[WOS] | Microbiology |
关键词[WOS] | SP STRAIN PCC-7120 ; PCC 7120 ; DIFFERENTIATION ; EXPRESSION ; SEQUENCE ; PATTERN ; GLYCOLIPIDS ; ENVELOPE |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000188841500023 |
公开日期 | 2010-10-13 |
源URL | [http://ir.ihb.ac.cn/handle/152342/9568] |
专题 | 水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文 |
作者单位 | Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
推荐引用方式 GB/T 7714 | Ning, DG,Xu, XD. alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120[J]. MICROBIOLOGY-SGM,2004,150(0):447-453. |
APA | Ning, DG,&Xu, XD.(2004).alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120.MICROBIOLOGY-SGM,150(0),447-453. |
MLA | Ning, DG,et al."alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120".MICROBIOLOGY-SGM 150.0(2004):447-453. |
入库方式: OAI收割
来源:水生生物研究所
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