alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120
文献类型：期刊论文
| 作者 | Ning, DG; Xu, XD |
| 刊名 | MICROBIOLOGY-SGM
 |
| 出版日期 | 2004-02-01 |
| 卷号 | 150期号:0页码:447-453 |
| 关键词 | CalA ftsZ DNA-binding protein Anabaena |
| ISSN号 | 1350-0872 |
| 通讯作者 | Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | Anabaena sp. PCC; 7120 was mutagenized by transposon Tn5-1087b, generating a mutant whose heterocysts lack the envelope polysaccharide layer. The transposon was located between nucleotides 342 and 343 of alr0117, a 918 bp gene encoding a histidine kinase for a two-component regulatory system. Complementation of the mutant with a DNA fragment containing alr0117 and targeted inactivation of the gene confirmed that alr0117 is involved in heterocyst development. RT-PCR showed that alr0117 was constitutively expressed in the presence or absence of a combined-nitrogen source. hepA and patB, the two genes turned on during wild-type heterocyst development, were no longer activated in an alr0117-null mutant. The two-component signal transduction system involving alr0117 may control the formation of the envelope polysaccharide layer and certain late events essential to the function of heterocysts. |
| 英文摘要 | Anabaena sp. PCC; 7120 was mutagenized by transposon Tn5-1087b, generating a mutant whose heterocysts lack the envelope polysaccharide layer. The transposon was located between nucleotides 342 and 343 of alr0117, a 918 bp gene encoding a histidine kinase for a two-component regulatory system. Complementation of the mutant with a DNA fragment containing alr0117 and targeted inactivation of the gene confirmed that alr0117 is involved in heterocyst development. RT-PCR showed that alr0117 was constitutively expressed in the presence or absence of a combined-nitrogen source. hepA and patB, the two genes turned on during wild-type heterocyst development, were no longer activated in an alr0117-null mutant. The two-component signal transduction system involving alr0117 may control the formation of the envelope polysaccharide layer and certain late events essential to the function of heterocysts. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Microbiology |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | SP STRAIN PCC-7120 ; PCC 7120 ; DIFFERENTIATION ; EXPRESSION ; SEQUENCE ; PATTERN ; GLYCOLIPIDS ; ENVELOPE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000188841500023 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9568]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ning, DG,Xu, XD. alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120[J]. MICROBIOLOGY-SGM,2004,150(0):447-453. |
| APA | Ning, DG,&Xu, XD.(2004).alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120.MICROBIOLOGY-SGM,150(0),447-453. |
| MLA | Ning, DG,et al."alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp PCC 7120".MICROBIOLOGY-SGM 150.0(2004):447-453. |
入库方式： OAI收割
来源：水生生物研究所
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