Molecular characterization and IFN signal pathway analysis of Carassius auratus CaSTAT1 identified from the cultured cells in response to virus infection
文献类型：期刊论文
| 作者 | Zhang, YB; Gui, HF |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2004-03-01 |
| 卷号 | 28期号:3页码:211-227 |
| 关键词 | Janus kinase-signal transducer and activator of transcription signal pathway STAT1 cDNA cloning expression analysis Crucian carp blastulae embryonic cells grass carp hemorrhagic virus |
| ISSN号 | 0145-305X |
| 通讯作者 | Gui, HF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Dept Fish Genet & Breeding, Inst Hydrobiol,Wuhan Ctr Dev Biol, Wuhan 430072, Peoples R China |
| 中文摘要 | Type I interferon (IFN) exerts its pleiotropic effects mainly through the JAK-STAT signaling pathway, which is presently best described in mammals. By subtractive suppression hybridization, two fish signaling factors, JAK1 and STAT1, had been identified in the IFN-induced crucian carp Carassius auratus L. blastulae embryonic (CAB) cells after treatment with UV-inactivated grass carp hemorrhagic virus (GCHV). Further, the full-length cDNA of STAT1, termed CaSTAT1, was obtained. It contains 2926 bp and encodes a protein of 718 aa. CaSTAT1 is most similar to rat STAT1 with 59% identity overall and displays all highly conserved domains that the STAT family possesses. Like human STAT1beta, it lacks the C-terminus acting as transcriptional activation domain in mammals. By contrast, only a single transcript was detected in virus-induced CAB cells. Expression analysis showed that CaSTAT1 could be activated by stimulation of CAB cells with poly I:C, active GCHV, UV-inactivated GCHV or CAB IFN, and displayed diverse expression patterns similar to that of mammalian STATI. Additionally, the expression of an antiviral gene CaMx1 was also induced under the same conditions, and expression difference between CaSTAT1 and CaMx1 was revealed by induction of CAB IFN. These results provide molecular evidence supporting the notion that the fish IFN signaling transduction pathway is similar to that in mammals. Fish IFN exerts its multiple functions, at least antiviral action, through a JAK-STAT pathway. (C) 2004 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Type I interferon (IFN) exerts its pleiotropic effects mainly through the JAK-STAT signaling pathway, which is presently best described in mammals. By subtractive suppression hybridization, two fish signaling factors, JAK1 and STAT1, had been identified in the IFN-induced crucian carp Carassius auratus L. blastulae embryonic (CAB) cells after treatment with UV-inactivated grass carp hemorrhagic virus (GCHV). Further, the full-length cDNA of STAT1, termed CaSTAT1, was obtained. It contains 2926 bp and encodes a protein of 718 aa. CaSTAT1 is most similar to rat STAT1 with 59% identity overall and displays all highly conserved domains that the STAT family possesses. Like human STAT1beta, it lacks the C-terminus acting as transcriptional activation domain in mammals. By contrast, only a single transcript was detected in virus-induced CAB cells. Expression analysis showed that CaSTAT1 could be activated by stimulation of CAB cells with poly I:C, active GCHV, UV-inactivated GCHV or CAB IFN, and displayed diverse expression patterns similar to that of mammalian STATI. Additionally, the expression of an antiviral gene CaMx1 was also induced under the same conditions, and expression difference between CaSTAT1 and CaMx1 was revealed by induction of CAB IFN. These results provide molecular evidence supporting the notion that the fish IFN signaling transduction pathway is similar to that in mammals. Fish IFN exerts its multiple functions, at least antiviral action, through a JAK-STAT pathway. (C) 2004 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Immunology; Zoology |
| 类目[WOS] | Immunology ; Zoology |
| 研究领域[WOS] | Immunology ; Zoology |
| 关键词[WOS] | PUFFERFISH TETRAODON-FLUVIATILIS ; INTERFERON REGULATORY FACTOR ; TRANSCRIPTION FACTOR ISGF-3 ; NF-KAPPA-B ; RAINBOW-TROUT ; GENE-EXPRESSION ; SERINE PHOSPHORYLATION ; GENOMIC ORGANIZATION ; TARGETED DISRUPTION ; STAT PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000188375700004 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9576]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Dept Fish Genet & Breeding, Inst Hydrobiol,Wuhan Ctr Dev Biol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, YB,Gui, HF. Molecular characterization and IFN signal pathway analysis of Carassius auratus CaSTAT1 identified from the cultured cells in response to virus infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2004,28(3):211-227. |
| APA | Zhang, YB,&Gui, HF.(2004).Molecular characterization and IFN signal pathway analysis of Carassius auratus CaSTAT1 identified from the cultured cells in response to virus infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,28(3),211-227. |
| MLA | Zhang, YB,et al."Molecular characterization and IFN signal pathway analysis of Carassius auratus CaSTAT1 identified from the cultured cells in response to virus infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 28.3(2004):211-227. |
入库方式： OAI收割
来源：水生生物研究所
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