Cloning of biologically active genomes from a Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus isolate by using a bacterial artificial chromosome
文献类型：期刊论文
| 作者 | Hu, ZH ; Chen, XW ; Sun, XL ; Vlak, JM ; Wang, HZ ; Deng, F ; Pijlman, GP |
| 刊名 | VIRUS RESEARCH
 |
| 出版日期 | 2003 |
| 卷号 | 97期号:2页码:57-63 |
| 关键词 | baculoviruses genotypes HaSNPV bacmid polyhedrin gene |
| 通讯作者 | Hu, ZH, Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China |
| 中文摘要 | Purification of genotypes from baculovirus isolates provides understanding of the diversity of baculoviruses and may lead to the development of better pesticides. Here, we report the cloning of different genotypes from an isolate of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HaSNPV) by using a bacterial artificial chromosome (BAC). A transfer vector (pHZB10) was constructed which contained an Escherichia coli mini-F replicon cassette within the upstream and downstream arms of HaSNPV polyhedrin gene. Hz2e5 cells were co-transfected with wild-type HaSNPV DNA and pHZB10 to generate recombinant viruses by homologous recombination. The DNA of budded viruses (BVs) was used to transform E. coli. One of the bacmid colonies, HaBacHZ8, has restriction enzyme digestion profiles similar to an in vivo cloned strain HaSNPV-G4, the genome of which has been completely sequenced. For testing the oral infectivity, the polyhedrin gene of HaSNPV was reintroduced into HaBacHZ8 to generate the recombinant bacmid HaBacDF6. The results of one-step growth curves, electron microscopic examination, protein expression analysis and bioassays indicated that HaBacDF6 replicated as well as HaSNPV-G4 in vitro and in vivo. The biologically functional HaSNPV bacmids obtained in this research will facilitate future studies on the function genomics and genetic modification of HaSNPV. (C) 2003 Elsevier B.V. All rights reserved. |
| 学科主题 | Virology |
| 收录类别 | SCI |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9598]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 推荐引用方式 GB/T 7714 | Hu, ZH,Chen, XW,Sun, XL,et al. Cloning of biologically active genomes from a Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus isolate by using a bacterial artificial chromosome[J]. VIRUS RESEARCH,2003,97(2):57-63. |
| APA | Hu, ZH.,Chen, XW.,Sun, XL.,Vlak, JM.,Wang, HZ.,...&Pijlman, GP.(2003).Cloning of biologically active genomes from a Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus isolate by using a bacterial artificial chromosome.VIRUS RESEARCH,97(2),57-63. |
| MLA | Hu, ZH,et al."Cloning of biologically active genomes from a Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus isolate by using a bacterial artificial chromosome".VIRUS RESEARCH 97.2(2003):57-63. |
入库方式： OAI收割
来源：水生生物研究所
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