Molecular cloning and characterization of crucian carp (Carassius auratus L.) interferon regulatory factor 7
文献类型：期刊论文
| 作者 | Zhang, YB; Hu, CY; Zhang, J; Huang, GP; Wei, LH; Zhang, QY; Gui, JF |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2003-11-01 |
| 卷号 | 15期号:5页码:453-466 |
| 关键词 | interferon regulatory factor (IRF) cDNA crucian carp blastulae embryonic (CAB) cells GCHV immune-related genes antiviral-relevant genes |
| ISSN号 | 1050-4648 |
| 通讯作者 | Gui, JF, Chinese Acad Sci, Inst Hydrobiol, Dept Fish Genet & Breeding, Wuhan 430072, Hubei Province, Peoples R China |
| 中文摘要 | Interferon (IFN) can induce an antiviral state via interferon-regulatory transcription factors (IRFs), which bind to and control genes directed by the interferon-stimulated response element (ISRE). Here we describe a fish IRF, termed CaIRF7, cloned from a subtractive cDNA library which is constructed with mRNAs obtained from crucian carp (Carassius auratus L.) blastulae embryonic (CAB) cells infected by UV-inactivated GCHV and mock-infected cells. CaIRF7 cDNA was found to be 1816 bp in length, with a 42 bp 5' UTR and a 508 bp 3' UTR. The open reading frame translates into 421 amino acids in which a DNA-binding domain (DBD) containing the repeated tryptophan motif and IRFs association domain have been identified. Like chicken GgIRF3, CaIRF7 was most similar to mammalian IRF7 with 27 to 30% identity overall and some 37% identity in their DBDs. A single transcript of 1.9 kb was detected in virally induced CAB cells by virtual Northern blotting. RT-PCR analysis revealed a wide tissue distribution of CaIRF7 constitutive expression, with detectable transcript in non-infected CAB cells and various tissues of healthy crucian carp. In addition, CaIRF7 expression was differentially increased by stimulation of the CAB cells with active GCHV, UV-inactivated GCHV or CAB IFN, indicating that the activation of CaIRF7 was directly regulated by IFN. (C) 2003 Published by Elsevier Ltd. |
| 英文摘要 | Interferon (IFN) can induce an antiviral state via interferon-regulatory transcription factors (IRFs), which bind to and control genes directed by the interferon-stimulated response element (ISRE). Here we describe a fish IRF, termed CaIRF7, cloned from a subtractive cDNA library which is constructed with mRNAs obtained from crucian carp (Carassius auratus L.) blastulae embryonic (CAB) cells infected by UV-inactivated GCHV and mock-infected cells. CaIRF7 cDNA was found to be 1816 bp in length, with a 42 bp 5' UTR and a 508 bp 3' UTR. The open reading frame translates into 421 amino acids in which a DNA-binding domain (DBD) containing the repeated tryptophan motif and IRFs association domain have been identified. Like chicken GgIRF3, CaIRF7 was most similar to mammalian IRF7 with 27 to 30% identity overall and some 37% identity in their DBDs. A single transcript of 1.9 kb was detected in virally induced CAB cells by virtual Northern blotting. RT-PCR analysis revealed a wide tissue distribution of CaIRF7 constitutive expression, with detectable transcript in non-infected CAB cells and various tissues of healthy crucian carp. In addition, CaIRF7 expression was differentially increased by stimulation of the CAB cells with active GCHV, UV-inactivated GCHV or CAB IFN, indicating that the activation of CaIRF7 was directly regulated by IFN. (C) 2003 Published by Elsevier Ltd. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | SEQUENCE-BINDING-PROTEIN ; TRANSCRIPTION ACTIVATION ; GENE INDUCTION ; ALPHA GENES ; EXPRESSION ; VIRUS ; DISTINCT ; FAMILY ; IRF-3 ; INFECTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000186734400008 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9610]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Wuhan 430072, Hubei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, YB,Hu, CY,Zhang, J,et al. Molecular cloning and characterization of crucian carp (Carassius auratus L.) interferon regulatory factor 7[J]. FISH & SHELLFISH IMMUNOLOGY,2003,15(5):453-466. |
| APA | Zhang, YB.,Hu, CY.,Zhang, J.,Huang, GP.,Wei, LH.,...&Gui, JF.(2003).Molecular cloning and characterization of crucian carp (Carassius auratus L.) interferon regulatory factor 7.FISH & SHELLFISH IMMUNOLOGY,15(5),453-466. |
| MLA | Zhang, YB,et al."Molecular cloning and characterization of crucian carp (Carassius auratus L.) interferon regulatory factor 7".FISH & SHELLFISH IMMUNOLOGY 15.5(2003):453-466. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。