Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp
文献类型：期刊论文
| 作者 | Dai, HP; Gao, H; Zhao, XY; Dai, LF; Zhang, XK; Xiao, N; Zhao, RH; Hemmingsen, SM |
| 刊名 | JOURNAL OF IMMUNOLOGICAL METHODS
 |
| 出版日期 | 2003-08-01 |
| 卷号 | 279期号:1-2页码:267-275 |
| 关键词 | phage display White Spot Syndrome Virus shrimp |
| ISSN号 | 0022-1759 |
| 通讯作者 | Dai, HP, Chinese Acad Sci, Inst Hydrobiol, E Lake So Rd 7 Wuchan, Wuhan 430072, Hubei, Peoples R China |
| 中文摘要 | An antibody phage display library against White Spot Syndrome Virus (WSSV) was constructed. After four rounds of panning against WSSV, 192 out of 480 clones displayed WSSV binding activity. One of the positive clones, designated A1, had relatively higher activity specifically binding to WSSV A1-soluble, single-chain fragment variable (scFv) antibody has an affinity constant (K-aff) of 2.02 +/- 0.42 x 10(9) M-1. Dot blot assays showed that A1-soluble scFv could detect WSSV directly from shrimp hemolymph after 24-h feeding infection by WSSV. A1 scFv has potential for the development of a cheap, simple and sensitive diagnostic kit for WSSV in the field. (C) 2003 Elsevier Science B.V. All rights reserved. |
| 英文摘要 | An antibody phage display library against White Spot Syndrome Virus (WSSV) was constructed. After four rounds of panning against WSSV, 192 out of 480 clones displayed WSSV binding activity. One of the positive clones, designated A1, had relatively higher activity specifically binding to WSSV A1-soluble, single-chain fragment variable (scFv) antibody has an affinity constant (K-aff) of 2.02 +/- 0.42 x 10(9) M-1. Dot blot assays showed that A1-soluble scFv could detect WSSV directly from shrimp hemolymph after 24-h feeding infection by WSSV. A1 scFv has potential for the development of a cheap, simple and sensitive diagnostic kit for WSSV in the field. (C) 2003 Elsevier Science B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemical Research Methods; Immunology |
| 类目[WOS] | Biochemical Research Methods ; Immunology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Immunology |
| 关键词[WOS] | PHAGE DISPLAY ; GENOME SEQUENCE ; PENAEID SHRIMP ; BACULOVIRUS ; POLYMERASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000185381300024 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9650]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 2.Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada |
| 推荐引用方式 GB/T 7714 | Dai, HP,Gao, H,Zhao, XY,et al. Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp[J]. JOURNAL OF IMMUNOLOGICAL METHODS,2003,279(1-2):267-275. |
| APA | Dai, HP.,Gao, H.,Zhao, XY.,Dai, LF.,Zhang, XK.,...&Hemmingsen, SM.(2003).Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp.JOURNAL OF IMMUNOLOGICAL METHODS,279(1-2),267-275. |
| MLA | Dai, HP,et al."Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp".JOURNAL OF IMMUNOLOGICAL METHODS 279.1-2(2003):267-275. |
入库方式： OAI收割
来源：水生生物研究所
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