Agrobacterium-mediated transformation of Kentucky bluegrass
文献类型：期刊论文
| 作者 | Chai, BF; Liang, AH; Wang, W; Wei, H |
| 刊名 | ACTA BOTANICA SINICA
 |
| 出版日期 | 2003-08-01 |
| 卷号 | 45期号:8页码:966-973 |
| 关键词 | monocotyledon callus Agrobacterium-mediation Poa pratensis |
| ISSN号 | 0577-7496 |
| 通讯作者 | Chai, BF, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | Embryogenic calli of Kentucky bluegrass, named Md, were induced from mature seeds and embryos, and proliferated on medium K3 containing 2,4-dichlorophenoxyacetic acid (2,4-D, 10.0 mumol/L), 6-benzylaminopurine (BAR, 0.5 mumol/L) and K5 which was the K3 medium supplemented with cupric sulfa (0.5 mumol/L) under dim-light condition (20-30 mumol.m(-2).s-1, 16 h light) at 24 degreesC. Embryogenic calli were transformed with plasmids pDM805 Carring bar and gus genes, Which was mediated by an Agrobacterium strain AGL1, four transgenic lines were obtained. The important factors that affect the transformation efficiency and obtain desirable number of transgenic plants included: (1) the quality of embryogenic calli; (2) light condition and time of co-cultivation; (3) concentration of antibiotics used for suppressing the overgrowth of Agrobacterium in the course of transformed plant regeneration; (4) selection pressure, etc. The micro nutrient of cupric had significant influence on the quality of embryogenic calli. This presentation is the first successful protocol of Kentucky bluegrass transformation mediated by Agrobacterium. |
| 英文摘要 | Embryogenic calli of Kentucky bluegrass, named Md, were induced from mature seeds and embryos, and proliferated on medium K3 containing 2,4-dichlorophenoxyacetic acid (2,4-D, 10.0 mumol/L), 6-benzylaminopurine (BAR, 0.5 mumol/L) and K5 which was the K3 medium supplemented with cupric sulfa (0.5 mumol/L) under dim-light condition (20-30 mumol.m(-2).s-1, 16 h light) at 24 degreesC. Embryogenic calli were transformed with plasmids pDM805 Carring bar and gus genes, Which was mediated by an Agrobacterium strain AGL1, four transgenic lines were obtained. The important factors that affect the transformation efficiency and obtain desirable number of transgenic plants included: (1) the quality of embryogenic calli; (2) light condition and time of co-cultivation; (3) concentration of antibiotics used for suppressing the overgrowth of Agrobacterium in the course of transformed plant regeneration; (4) selection pressure, etc. The micro nutrient of cupric had significant influence on the quality of embryogenic calli. This presentation is the first successful protocol of Kentucky bluegrass transformation mediated by Agrobacterium. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Plant Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Plant Sciences |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Plant Sciences |
| 关键词[WOS] | T-DNA ; RICE ; GENE ; TUMEFACIENS ; EXPRESSION ; CULTURES ; REGION ; MARKER ; PLANTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000184913500013 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9666]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Shanxi Univ, Inst Biotechnol, Taiyuan 030006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chai, BF,Liang, AH,Wang, W,et al. Agrobacterium-mediated transformation of Kentucky bluegrass[J]. ACTA BOTANICA SINICA,2003,45(8):966-973. |
| APA | Chai, BF,Liang, AH,Wang, W,&Wei, H.(2003).Agrobacterium-mediated transformation of Kentucky bluegrass.ACTA BOTANICA SINICA,45(8),966-973. |
| MLA | Chai, BF,et al."Agrobacterium-mediated transformation of Kentucky bluegrass".ACTA BOTANICA SINICA 45.8(2003):966-973. |
入库方式： OAI收割
来源：水生生物研究所
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