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Gene transfer into genomes of human cells by the sleeping beauty transposon system
文献类型：期刊论文
| 作者 | Geurts, AM; Yang, Y; Clark, KJ; Liu, GY; Cui, ZB; Dupuy, AJ; Bell, JB; Largaespada, DA; Hackett, PB |
| 刊名 | MOLECULAR THERAPY
 |
| 出版日期 | 2003-07-01 |
| 卷号 | 8期号:1页码:108-117 |
| ISSN号 | 1525-0016 |
| 关键词 | gene therapy inverted terminal repeats site-specific mutagenesis transposase |
| 通讯作者 | Geurts, AM, Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA |
| 中文摘要 | The Sleeping Beauty (SB) transposon system, derived from teleost fish sequences, is extremely effective at delivering DNA to vertebrate genomes, including those of humans. We have examined several parameters of the SB system to improve it as a potential, nonviral vector for gene therapy. Our investigation centered on three features: the carrying capacity of the transposon for efficient integration into chromosomes of HeLa cells, the effects of overexpression of the SB transposase gene on transposition rates, and improvements in the activity of SB transposase to increase insertion rates of transgenes into cellular chromosomes. We found that SB transposons of about 6 kb retained 50% of the maximal efficiency of transposition, which is sufficient to deliver 70-80% of identified human cDNAs with appropriate transcriptional regulatory sequences. Overexpression inhibition studies revealed that there are optimal ratios of SB transposase to transposon for maximal rates of transposition, suggesting that conditions of delivery of the two-part transposon system are important for the best gene-transfer efficiencies. We further refined the SB transposase to incorporate several amino acid substitutions, the result of which led to an improved transposase called SB11. With SB11 we are able to achieve transposition rates that are about 100-fold above those achieved with plasmids that insert into chromosomes by random recombination. With the recently described improvements to the transposon itself, the SB system appears to be a potential gene-transfer tool for human gene therapy. |
| 英文摘要 | The Sleeping Beauty (SB) transposon system, derived from teleost fish sequences, is extremely effective at delivering DNA to vertebrate genomes, including those of humans. We have examined several parameters of the SB system to improve it as a potential, nonviral vector for gene therapy. Our investigation centered on three features: the carrying capacity of the transposon for efficient integration into chromosomes of HeLa cells, the effects of overexpression of the SB transposase gene on transposition rates, and improvements in the activity of SB transposase to increase insertion rates of transgenes into cellular chromosomes. We found that SB transposons of about 6 kb retained 50% of the maximal efficiency of transposition, which is sufficient to deliver 70-80% of identified human cDNAs with appropriate transcriptional regulatory sequences. Overexpression inhibition studies revealed that there are optimal ratios of SB transposase to transposon for maximal rates of transposition, suggesting that conditions of delivery of the two-part transposon system are important for the best gene-transfer efficiencies. We further refined the SB transposase to incorporate several amino acid substitutions, the result of which led to an improved transposase called SB11. With SB11 we are able to achieve transposition rates that are about 100-fold above those achieved with plasmids that insert into chromosomes by random recombination. With the recently described improvements to the transposon itself, the SB system appears to be a potential gene-transfer tool for human gene therapy. |
| 学科主题 | Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine ; Research & Experimental |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine |
| 关键词[WOS] | CHROMOSOMAL TRANSPOSITION ; TRANSGENE EXPRESSION ; PLASMID DNA ; GERM-LINE ; THERAPY ; ELEMENTS ; VECTOR ; MOUSE ; DELIVERY ; FISH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000184066900017 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9684]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA 2.Univ Minnesota, Arnold & Mabel Beckman Ctr Transposon Res, Minneapolis, MN 55455 USA 3.Discovery Genom Inc, Minneapolis, MN 55413 USA 4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Geurts, AM,Yang, Y,Clark, KJ,et al. Gene transfer into genomes of human cells by the sleeping beauty transposon system[J]. MOLECULAR THERAPY,2003,8(1):108-117. |
| APA | Geurts, AM.,Yang, Y.,Clark, KJ.,Liu, GY.,Cui, ZB.,...&Hackett, PB.(2003).Gene transfer into genomes of human cells by the sleeping beauty transposon system.MOLECULAR THERAPY,8(1),108-117. |
| MLA | Geurts, AM,et al."Gene transfer into genomes of human cells by the sleeping beauty transposon system".MOLECULAR THERAPY 8.1(2003):108-117. |
入库方式： OAI收割
来源：水生生物研究所
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