中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody

文献类型:期刊论文

作者Lu, YS; Nie, P; Sun, BJ
刊名DISEASES OF AQUATIC ORGANISMS
出版日期2003-03-31
卷号54期号:2页码:171-173
关键词myxosporean Myxobolus rotundus crucian carp Carassius auratus auratus skin mucus monoclonal antibody diagnosis
ISSN号0177-5103
通讯作者Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要Diagnosis of myxosporean Myxobolus rotundus infection was conducted by examining skin mucus from the infected crucian carp Carassius auratus auratus with a monoclonal antibody, MAb 2D12, raised previously against the parasite. A positive reaction was observed in skin mucus collected from infected fish, and spores and pre-spore stages of the parasite were identified by the MAb 2D12. It was also demonstrated that M. rotundus infection can be successfully detected by a simple method, enzyme-linked immunosorbent assay (ELISA), and that skin mucus collected from infected fish skin had a significantly higher optical density (OD) value than that from uninfected fish.
英文摘要Diagnosis of myxosporean Myxobolus rotundus infection was conducted by examining skin mucus from the infected crucian carp Carassius auratus auratus with a monoclonal antibody, MAb 2D12, raised previously against the parasite. A positive reaction was observed in skin mucus collected from infected fish, and spores and pre-spore stages of the parasite were identified by the MAb 2D12. It was also demonstrated that M. rotundus infection can be successfully detected by a simple method, enzyme-linked immunosorbent assay (ELISA), and that skin mucus collected from infected fish skin had a significantly higher optical density (OD) value than that from uninfected fish.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科主题Fisheries; Veterinary Sciences
类目[WOS]Fisheries ; Veterinary Sciences
研究领域[WOS]Fisheries ; Veterinary Sciences
关键词[WOS]MYXOSOMA-CEREBRALIS ; RAINBOW-TROUT ; DISEASE ; FISH
收录类别SCI
语种英语
WOS记录号WOS:000183491500010
公开日期2010-10-13
源URL[http://ir.ihb.ac.cn/handle/152342/9690]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文
作者单位1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Lab Fish Dis, Inst Hydrol, Wuhan 430072, Peoples R China
推荐引用方式
GB/T 7714
Lu, YS,Nie, P,Sun, BJ. Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody[J]. DISEASES OF AQUATIC ORGANISMS,2003,54(2):171-173.
APA Lu, YS,Nie, P,&Sun, BJ.(2003).Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody.DISEASES OF AQUATIC ORGANISMS,54(2),171-173.
MLA Lu, YS,et al."Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody".DISEASES OF AQUATIC ORGANISMS 54.2(2003):171-173.

入库方式: OAI收割

来源:水生生物研究所

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