Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody
文献类型:期刊论文
作者 | Lu, YS; Nie, P; Sun, BJ |
刊名 | DISEASES OF AQUATIC ORGANISMS
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出版日期 | 2003-03-31 |
卷号 | 54期号:2页码:171-173 |
关键词 | myxosporean Myxobolus rotundus crucian carp Carassius auratus auratus skin mucus monoclonal antibody diagnosis |
ISSN号 | 0177-5103 |
通讯作者 | Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
中文摘要 | Diagnosis of myxosporean Myxobolus rotundus infection was conducted by examining skin mucus from the infected crucian carp Carassius auratus auratus with a monoclonal antibody, MAb 2D12, raised previously against the parasite. A positive reaction was observed in skin mucus collected from infected fish, and spores and pre-spore stages of the parasite were identified by the MAb 2D12. It was also demonstrated that M. rotundus infection can be successfully detected by a simple method, enzyme-linked immunosorbent assay (ELISA), and that skin mucus collected from infected fish skin had a significantly higher optical density (OD) value than that from uninfected fish. |
英文摘要 | Diagnosis of myxosporean Myxobolus rotundus infection was conducted by examining skin mucus from the infected crucian carp Carassius auratus auratus with a monoclonal antibody, MAb 2D12, raised previously against the parasite. A positive reaction was observed in skin mucus collected from infected fish, and spores and pre-spore stages of the parasite were identified by the MAb 2D12. It was also demonstrated that M. rotundus infection can be successfully detected by a simple method, enzyme-linked immunosorbent assay (ELISA), and that skin mucus collected from infected fish skin had a significantly higher optical density (OD) value than that from uninfected fish. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
学科主题 | Fisheries; Veterinary Sciences |
类目[WOS] | Fisheries ; Veterinary Sciences |
研究领域[WOS] | Fisheries ; Veterinary Sciences |
关键词[WOS] | MYXOSOMA-CEREBRALIS ; RAINBOW-TROUT ; DISEASE ; FISH |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000183491500010 |
公开日期 | 2010-10-13 |
源URL | [http://ir.ihb.ac.cn/handle/152342/9690] ![]() |
专题 | 水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文 |
作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Lab Fish Dis, Inst Hydrol, Wuhan 430072, Peoples R China |
推荐引用方式 GB/T 7714 | Lu, YS,Nie, P,Sun, BJ. Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody[J]. DISEASES OF AQUATIC ORGANISMS,2003,54(2):171-173. |
APA | Lu, YS,Nie, P,&Sun, BJ.(2003).Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody.DISEASES OF AQUATIC ORGANISMS,54(2),171-173. |
MLA | Lu, YS,et al."Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody".DISEASES OF AQUATIC ORGANISMS 54.2(2003):171-173. |
入库方式: OAI收割
来源:水生生物研究所
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