Culture of the terrestrial cyanobacterium, Nostoc flagelliforme (Cyanophyceae), under aquatic conditions
文献类型：期刊论文
| 作者 | Gao, KS; Ye, CP |
| 刊名 | JOURNAL OF PHYCOLOGY
 |
| 出版日期 | 2003-06-01 |
| 卷号 | 39期号:3页码:617-623 |
| 关键词 | blue-green algae colonial filament cyanobacteria desiccation Nostoc flagelliforme sheath disintegration single filament |
| ISSN号 | 0022-3646 |
| 通讯作者 | Gao, KS, Shantou Univ, Ctr Sci, Inst Marine Biol, Shantou 515063, Guangdong, Peoples R China |
| 中文摘要 | Both colonies and free-living cells of the terrestrial cyanobacterium, Nostoc flagelliforme (Berk. & Curtis) Bornet & Flahault, were cultured under aquatic conditions to develop the techniques for the cultivation and restoration of this endangered resource. The colonial filaments disintegrated with their sheaths ruptured in about 2 days without any desiccating treatments. Periodic desiccation played an important role in preventing the alga from decomposing, with greater delays to sheath rupture with a higher frequency of exposure to air. The bacterial numbers in the culture treated with seven periods of desiccation per day were about 50% less compared with the cultures without the desiccation treatment. When bacteria in the culture were controlled, the colonial filaments did not disintegrate and maintained the integrity of their sheath for about 20 days even without the desiccation treatments, indicating the importance of desiccation for N. flagelliforme to prevent them from being disintegrated by bacteria. On the other hand, when free-living cells obtained from crushed colonial filaments were cultured in liquid medium, they developed into single filaments with sheaths, within which multiple filaments were formed later on as a colony. Such colonial filaments were developed at 15, 25, and 30degreesC at either 20 or 60 mumol photons.m(-2).s(-1); colonies did not develop at 180 mumol photons.m(-2).s(-1), though this light level resulted in the most rapid growth of the cells. Conditions of 60 mumol photons.m(-2).s(-1) and 25degrees C appeared to result in the best colonial development and faster growth of the sheath-held colonies of N. flagelliforme when cultured indoor under aquatic conditions. |
| 英文摘要 | Both colonies and free-living cells of the terrestrial cyanobacterium, Nostoc flagelliforme (Berk. & Curtis) Bornet & Flahault, were cultured under aquatic conditions to develop the techniques for the cultivation and restoration of this endangered resource. The colonial filaments disintegrated with their sheaths ruptured in about 2 days without any desiccating treatments. Periodic desiccation played an important role in preventing the alga from decomposing, with greater delays to sheath rupture with a higher frequency of exposure to air. The bacterial numbers in the culture treated with seven periods of desiccation per day were about 50% less compared with the cultures without the desiccation treatment. When bacteria in the culture were controlled, the colonial filaments did not disintegrate and maintained the integrity of their sheath for about 20 days even without the desiccation treatments, indicating the importance of desiccation for N. flagelliforme to prevent them from being disintegrated by bacteria. On the other hand, when free-living cells obtained from crushed colonial filaments were cultured in liquid medium, they developed into single filaments with sheaths, within which multiple filaments were formed later on as a colony. Such colonial filaments were developed at 15, 25, and 30degreesC at either 20 or 60 mumol photons.m(-2).s(-1); colonies did not develop at 180 mumol photons.m(-2).s(-1), though this light level resulted in the most rapid growth of the cells. Conditions of 60 mumol photons.m(-2).s(-1) and 25degrees C appeared to result in the best colonial development and faster growth of the sheath-held colonies of N. flagelliforme when cultured indoor under aquatic conditions. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Plant Sciences; Marine & Freshwater Biology |
| 类目[WOS] | Plant Sciences ; Marine & Freshwater Biology |
| 研究领域[WOS] | Plant Sciences ; Marine & Freshwater Biology |
| 关键词[WOS] | BLUE-GREEN-ALGA ; PHOTOSYNTHETIC CHARACTERISTICS ; EXTRACELLULAR INVESTMENT ; STRUCTURAL MODIFICATIONS ; COMMUNE CYANOBACTERIA ; LIFE-CYCLE ; DESICCATION ; PURIFICATION ; RECOVERY ; VAUCH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000183070000019 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9698]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Shantou Univ, Ctr Sci, Inst Marine Biol, Shantou 515063, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, KS,Ye, CP. Culture of the terrestrial cyanobacterium, Nostoc flagelliforme (Cyanophyceae), under aquatic conditions[J]. JOURNAL OF PHYCOLOGY,2003,39(3):617-623. |
| APA | Gao, KS,&Ye, CP.(2003).Culture of the terrestrial cyanobacterium, Nostoc flagelliforme (Cyanophyceae), under aquatic conditions.JOURNAL OF PHYCOLOGY,39(3),617-623. |
| MLA | Gao, KS,et al."Culture of the terrestrial cyanobacterium, Nostoc flagelliforme (Cyanophyceae), under aquatic conditions".JOURNAL OF PHYCOLOGY 39.3(2003):617-623. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。