RecA-mediated, targeted mutagenesis in zebrafish
文献类型：期刊论文
| 作者 | Cui, ZB; Yang, Y; Kaufman, CD; Agalliu, D; Hackett, PB |
| 刊名 | MARINE BIOTECHNOLOGY
 |
| 出版日期 | 2003-03-01 |
| 卷号 | 5期号:2页码:174-184 |
| 关键词 | RecA protein gene targeting site-specific mutagenesis zebrafish |
| ISSN号 | 1436-2228 |
| 通讯作者 | Yang, Y, Univ Minnesota, Dept Genet Cell Biol & Dev, St Paul, MN 55108 USA |
| 中文摘要 | We have evaluated the efficacy of RecA, a prokaryotic protein involved with homologous recombination, to direct site-specific mutagenesis in zebrafish embryos. For this we coinjected a vector containing a mutated enhanced green fluorescent protein (EGFP) gene plus 236-nucleotide corrective single-stranded DNAs coated with RecA into I-cell zebrafish embryos. Twenty-hours after fertilization, about 5% to 20% of injected embryos showed EGFP expression in I or more cells when RecA-coated corrective DNAs were used, but not when RecA was omitted. Mutated EGFP genes with 1-bp insertions or deletions were inefficiently activated, whereas those with 7-bp insertions were activated about 4-fold more efficiently. RecA-coated template strand had a higher efficiency than its complementary strand in activation of EGFP expression. Prior irradiation of the embryos with UV light enhanced RecA-mediated restoration of gene activity, suggesting that the effects we observed were augmented by one or more factors of zebrafish DNA repair systems. |
| 英文摘要 | We have evaluated the efficacy of RecA, a prokaryotic protein involved with homologous recombination, to direct site-specific mutagenesis in zebrafish embryos. For this we coinjected a vector containing a mutated enhanced green fluorescent protein (EGFP) gene plus 236-nucleotide corrective single-stranded DNAs coated with RecA into I-cell zebrafish embryos. Twenty-hours after fertilization, about 5% to 20% of injected embryos showed EGFP expression in I or more cells when RecA-coated corrective DNAs were used, but not when RecA was omitted. Mutated EGFP genes with 1-bp insertions or deletions were inefficiently activated, whereas those with 7-bp insertions were activated about 4-fold more efficiently. RecA-coated template strand had a higher efficiency than its complementary strand in activation of EGFP expression. Prior irradiation of the embryos with UV light enhanced RecA-mediated restoration of gene activity, suggesting that the effects we observed were augmented by one or more factors of zebrafish DNA repair systems. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biotechnology & Applied Microbiology; Marine & Freshwater Biology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 关键词[WOS] | STIMULATES HOMOLOGOUS RECOMBINATION ; DNA STRAND-EXCHANGE ; TRANSGENIC ZEBRAFISH ; ESCHERICHIA-COLI ; ORYZIAS-LATIPES ; MAMMALIAN-CELLS ; PROTEIN ; REPAIR ; GENE ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000182588700011 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9700]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Univ Minnesota, Dept Genet Cell Biol & Dev, St Paul, MN 55108 USA 2.Univ Minnesota, Arnold & Mabel Beckman Ctr Transposon Res, St Paul, MN 55108 USA 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, ZB,Yang, Y,Kaufman, CD,et al. RecA-mediated, targeted mutagenesis in zebrafish[J]. MARINE BIOTECHNOLOGY,2003,5(2):174-184. |
| APA | Cui, ZB,Yang, Y,Kaufman, CD,Agalliu, D,&Hackett, PB.(2003).RecA-mediated, targeted mutagenesis in zebrafish.MARINE BIOTECHNOLOGY,5(2),174-184. |
| MLA | Cui, ZB,et al."RecA-mediated, targeted mutagenesis in zebrafish".MARINE BIOTECHNOLOGY 5.2(2003):174-184. |
入库方式： OAI收割
来源：水生生物研究所
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