Identification of antiviral-relevant genes in the cultured fish cells induced by UV-inactivated virus
文献类型：期刊论文
| 作者 | Zhang, YB; Zhang, QY; Xu, DQ; Hu, CY; Gui, JF |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2003-03-01 |
| 卷号 | 48期号:6页码:581-588 |
| 关键词 | antiviral-relevant genes immune-relevant genes grass carp hemorrhage virus (GCHV) interferon suppressive subtractive hybridization |
| ISSN号 | 1001-6538 |
| 通讯作者 | Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | UV-inactivated grass carp hemorrhage virus (GCHV) can induce high titer of interferon in cultured CAB (crucian carp (Carassius auratus L.) blastulae) cells, and thus defend host cells against the virus invasion. The mechanism is proposed that an antiviral state should be established in the host cells by activating expression of a set of antiviral-relevant genes. In this study, suppressive subtractive hybridization is applied to constructing a subtracted cDNA library with mRNAs isolated from UV-inactivated GCHV infected and mock-infected CAB cells. 272 differential cDNA fragments are identified by both PCR and dot blot from the subtractive cDNA library. Sequencing analysis reveals 69 genes, including 46 known gene homologues, and 23 unknown putative genes. The known genes include the genes involved in interferon signaling pathways, such as Stat1 and Jak1, the antiviral genes, such as Mx and Viperin, and a set of interferon-stimulated genes observed in mammalian cells. Most of the unknown putative genes contain AU-rich element in their sequences. Differential expressions of these genes are further confirmed by virtual Northern blot and RT-PCR. The data imply that UV-inactivated GCHV is not only able to induce production of interferon in the infected CAB cells, but also leads to the expression of a series of antiviral-relevant genes or immune-relevant genes, and therefore reveals that the signaling pathway of interferon system and antiviral mechanism in fish are similar to those in mammals. |
| 英文摘要 | UV-inactivated grass carp hemorrhage virus (GCHV) can induce high titer of interferon in cultured CAB (crucian carp (Carassius auratus L.) blastulae) cells, and thus defend host cells against the virus invasion. The mechanism is proposed that an antiviral state should be established in the host cells by activating expression of a set of antiviral-relevant genes. In this study, suppressive subtractive hybridization is applied to constructing a subtracted cDNA library with mRNAs isolated from UV-inactivated GCHV infected and mock-infected CAB cells. 272 differential cDNA fragments are identified by both PCR and dot blot from the subtractive cDNA library. Sequencing analysis reveals 69 genes, including 46 known gene homologues, and 23 unknown putative genes. The known genes include the genes involved in interferon signaling pathways, such as Stat1 and Jak1, the antiviral genes, such as Mx and Viperin, and a set of interferon-stimulated genes observed in mammalian cells. Most of the unknown putative genes contain AU-rich element in their sequences. Differential expressions of these genes are further confirmed by virtual Northern blot and RT-PCR. The data imply that UV-inactivated GCHV is not only able to induce production of interferon in the infected CAB cells, but also leads to the expression of a series of antiviral-relevant genes or immune-relevant genes, and therefore reveals that the signaling pathway of interferon system and antiviral mechanism in fish are similar to those in mammals. |
| WOS标题词 | Science & Technology |
| 学科主题 | Multidisciplinary Sciences |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | DOUBLE-STRANDED-RNA ; PARALICHTHYS-OLIVACEUS ; ONCORHYNCHUS-MYKISS ; JAPANESE FLOUNDER ; MOLECULAR-CLONING ; RAINBOW-TROUT ; EXPRESSION ; FAMILY ; CARP |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000182036500017 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9718]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, YB,Zhang, QY,Xu, DQ,et al. Identification of antiviral-relevant genes in the cultured fish cells induced by UV-inactivated virus[J]. CHINESE SCIENCE BULLETIN,2003,48(6):581-588. |
| APA | Zhang, YB,Zhang, QY,Xu, DQ,Hu, CY,&Gui, JF.(2003).Identification of antiviral-relevant genes in the cultured fish cells induced by UV-inactivated virus.CHINESE SCIENCE BULLETIN,48(6),581-588. |
| MLA | Zhang, YB,et al."Identification of antiviral-relevant genes in the cultured fish cells induced by UV-inactivated virus".CHINESE SCIENCE BULLETIN 48.6(2003):581-588. |
入库方式： OAI收割
来源：水生生物研究所
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