中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Determination of some reactive oxygen species scavengers based on the peroxidase-oxidase oscillator

文献类型:期刊论文

作者Gan, NQ; Cai, RX; Lin, ZX; Song, LR
刊名ANALYST
出版日期2003
卷号128期号:3页码:301-305
关键词MECHANISM
ISSN号0003-2654
通讯作者Cai, RX, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要This paper reports a new method for detection of ROS scavengers including superoxide dismutase, ascorbic acid and glutathione based on a 'probe' of peroxidase-oxidase biochemical oscillator. The oscillation period and amplitude change with different concentrations of scavengers. The linear ranges of superoxide dismutase, ascorbic acid and glutathione are respectively 1.56 x 10(-4)-1.56 x 10(-3) mg mL(-1), 1.75 x 10(-7) -1.75 x 10(-5) mol L-1 and 9.38 x 10(-7) -7.5 x 10(-5) mol L-1. The selectivity, linearity and precision for superoxide dismutase, ascorbic acid, and glutathione are presented and discussed. The results compared well with other standard methods for determination of superoxide dismutase, ascorbic acid and glutathione. Some possible steps in the overall reaction mechanisms are discussed.
英文摘要This paper reports a new method for detection of ROS scavengers including superoxide dismutase, ascorbic acid and glutathione based on a 'probe' of peroxidase-oxidase biochemical oscillator. The oscillation period and amplitude change with different concentrations of scavengers. The linear ranges of superoxide dismutase, ascorbic acid and glutathione are respectively 1.56 x 10(-4)-1.56 x 10(-3) mg mL(-1), 1.75 x 10(-7) -1.75 x 10(-5) mol L-1 and 9.38 x 10(-7) -7.5 x 10(-5) mol L-1. The selectivity, linearity and precision for superoxide dismutase, ascorbic acid, and glutathione are presented and discussed. The results compared well with other standard methods for determination of superoxide dismutase, ascorbic acid and glutathione. Some possible steps in the overall reaction mechanisms are discussed.
WOS标题词Science & Technology ; Physical Sciences
学科主题Chemistry ; Analytical
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MECHANISM ; CHAOS
收录类别SCI
语种英语
WOS记录号WOS:000181357900017
公开日期2010-10-13
源URL[http://ir.ihb.ac.cn/handle/152342/9740]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文
作者单位1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
推荐引用方式
GB/T 7714
Gan, NQ,Cai, RX,Lin, ZX,et al. Determination of some reactive oxygen species scavengers based on the peroxidase-oxidase oscillator[J]. ANALYST,2003,128(3):301-305.
APA Gan, NQ,Cai, RX,Lin, ZX,&Song, LR.(2003).Determination of some reactive oxygen species scavengers based on the peroxidase-oxidase oscillator.ANALYST,128(3),301-305.
MLA Gan, NQ,et al."Determination of some reactive oxygen species scavengers based on the peroxidase-oxidase oscillator".ANALYST 128.3(2003):301-305.

入库方式: OAI收割

来源:水生生物研究所

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