Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples
文献类型：期刊论文
| 作者 | Pan, H; Song, LR; Liu, YD; Borner, T |
| 刊名 | ARCHIVES OF MICROBIOLOGY
 |
| 出版日期 | 2002-12-01 |
| 卷号 | 178期号:6页码:421-427 |
| 关键词 | cyanobacteria Microcystis microcystin toxin peptide synthetase whole-cell PCR |
| ISSN号 | 0302-8933 |
| 通讯作者 | Song, LR, Chinese Acad Sci, Inst Hydrobiol, Dept Phycol, Loujiashan, Wuhan 430072, Peoples R China |
| 中文摘要 | Microcystins are small hepatotoxic peptides produced by a number of cyanobacteria. They are synthesized non-ribosomally by multifunctional enzyme complex synthetases encoded by the mcy genes. Primers deduced from mcy genes were designed to discriminate between toxic microcystin-producing strains and non-toxic strains. Thus, PCR-mediated detection of mcy genes could be a simple and efficient means to identify potentially harmful genotypes among cyanobacterial populations in bodies of water. We surveyed the distribution of the mcyB gene in different Microcystis strains isolated from Chinese bodies of water and confirmed that PCR can be reliably used to identify toxic strains. By omitting any DNA purification steps, the modified PCR protocol can greatly simplify the process. Cyanobacterial cells enriched from cultures, field samples, or even sediment samples could be used in the PCR assay. This method proved sensitive enough to detect mcyB genes in samples with less than 2,000 Microcystis cells per ml. Its accuracy, specificity and applicability were confirmed by sequencing selected DNA amplicons, as well as by HPLC, ELISA and mouse bioassay as controls for toxin production of every strain used. |
| 英文摘要 | Microcystins are small hepatotoxic peptides produced by a number of cyanobacteria. They are synthesized non-ribosomally by multifunctional enzyme complex synthetases encoded by the mcy genes. Primers deduced from mcy genes were designed to discriminate between toxic microcystin-producing strains and non-toxic strains. Thus, PCR-mediated detection of mcy genes could be a simple and efficient means to identify potentially harmful genotypes among cyanobacterial populations in bodies of water. We surveyed the distribution of the mcyB gene in different Microcystis strains isolated from Chinese bodies of water and confirmed that PCR can be reliably used to identify toxic strains. By omitting any DNA purification steps, the modified PCR protocol can greatly simplify the process. Cyanobacterial cells enriched from cultures, field samples, or even sediment samples could be used in the PCR assay. This method proved sensitive enough to detect mcyB genes in samples with less than 2,000 Microcystis cells per ml. Its accuracy, specificity and applicability were confirmed by sequencing selected DNA amplicons, as well as by HPLC, ELISA and mouse bioassay as controls for toxin production of every strain used. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Microbiology |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | POLYMERASE CHAIN-REACTION ; PEPTIDE SYNTHETASE GENES ; CYCLIC HEPTAPEPTIDE MICROCYSTIN ; DNA ; AMPLIFICATION ; BIOSYNTHESIS ; EXTRACTION ; SEDIMENTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000179674200006 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9766]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Dept Phycol, Wuhan 430072, Peoples R China 2.Humboldt Univ, Inst Biol, D-10115 Berlin, Germany |
| 推荐引用方式 GB/T 7714 | Pan, H,Song, LR,Liu, YD,et al. Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples[J]. ARCHIVES OF MICROBIOLOGY,2002,178(6):421-427. |
| APA | Pan, H,Song, LR,Liu, YD,&Borner, T.(2002).Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples.ARCHIVES OF MICROBIOLOGY,178(6),421-427. |
| MLA | Pan, H,et al."Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples".ARCHIVES OF MICROBIOLOGY 178.6(2002):421-427. |
入库方式： OAI收割
来源：水生生物研究所
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