Nuclear transplantation in different strains of zebrafish
文献类型：期刊论文
| 作者 | Hu, W; Wang, YP; Chen, SP; Zhu, ZY |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2002-08-01 |
| 卷号 | 47期号:15页码:1277-1280 |
| 关键词 | zebrafish different strains nuclear transplantation DNA fingerprint |
| ISSN号 | 1001-6538 |
| 通讯作者 | Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Single later blastula nuclei from AB strain of zebrafish (Danio rerio) were transplanted into enucleated unfertilized eggs of Long fin strain. Of 1119 cloning embryos, 14 reconstructed embryos developed into fry. DNA fingerprinting systems of the cloned fish were similar to those of the nuclear donor fish, but were distinctly different from those of the unclear recipient fish. It confirmed that the genetic material originated from nuclear donor cell other than from nuclear recipient egg. The research suggested that the basic technique for nuclear transplantation performed with different strains of zebrafish has made a breakthrough. It should be helpful for the study of some important developmental problems such as gene function, the regulation of gene expression during animal development, the developmental potential of a nucleus and the interactions between the donor nucleus and the recipient cytoplasm, etc. |
| 英文摘要 | Single later blastula nuclei from AB strain of zebrafish (Danio rerio) were transplanted into enucleated unfertilized eggs of Long fin strain. Of 1119 cloning embryos, 14 reconstructed embryos developed into fry. DNA fingerprinting systems of the cloned fish were similar to those of the nuclear donor fish, but were distinctly different from those of the unclear recipient fish. It confirmed that the genetic material originated from nuclear donor cell other than from nuclear recipient egg. The research suggested that the basic technique for nuclear transplantation performed with different strains of zebrafish has made a breakthrough. It should be helpful for the study of some important developmental problems such as gene function, the regulation of gene expression during animal development, the developmental potential of a nucleus and the interactions between the donor nucleus and the recipient cytoplasm, etc. |
| WOS标题词 | Science & Technology |
| 学科主题 | Multidisciplinary Sciences |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | ORYZIAS-LATIPES ; EMBRYOS ; MEDAKA ; CELLS ; EGGS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000177045600009 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9826]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, W,Wang, YP,Chen, SP,et al. Nuclear transplantation in different strains of zebrafish[J]. CHINESE SCIENCE BULLETIN,2002,47(15):1277-1280. |
| APA | Hu, W,Wang, YP,Chen, SP,&Zhu, ZY.(2002).Nuclear transplantation in different strains of zebrafish.CHINESE SCIENCE BULLETIN,47(15),1277-1280. |
| MLA | Hu, W,et al."Nuclear transplantation in different strains of zebrafish".CHINESE SCIENCE BULLETIN 47.15(2002):1277-1280. |
入库方式： OAI收割
来源：水生生物研究所
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