Development of polymorphic EST markers in Penaeus monodon: applications in penaeid genetics
文献类型：期刊论文
| 作者 | Tong, J; Lehnert, SA; Byrne, K; Kwan, HS; Chu, KH |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2002-05-31 |
| 卷号 | 208期号:1-2页码:69-79 |
| 关键词 | expressed sequence tags Penaeus monodon genome mapping shrimp |
| ISSN号 | 0044-8486 |
| 通讯作者 | Chu, KH, Chinese Univ Hong Kong, Dept Biol, Shatin, Hong Kong, Peoples R China |
| 中文摘要 | The objective of this study was to develop type I markers for genome mapping and other genetic studies of Penaeus monodon. Primers were designed based on expressed sequence tags (ESTs) from a P monodon cephalothorax cDNA library to amplify 100-300 bp products. 34 of the primer pairs successfully amplified PCR products from genomic DNA. Single-strand conformation polymorphism analysis showed that similar to 30% of the ESTs tested exhibit polymorphism in a test panel of P monodon individuals. Mendelian inheritance of the EST-derived markers has been established in two international reference mapping families of P monodon, and mapping of these markers is in progress. Some ESTs were successfully amplified from other Penaeus species (P. chinensis, P japonicus and P vannamei), indicating that the markers are applicable in cross-species comparison. Two populations of P. japonicus could be differentiated using one of the ESTS. In conclusion, the polymorphic EST markers developed in this study are applicable in genome mapping and population genetic studies of penaeid shrimp. (C) 2002 Elsevier Science B.V. All rights reserved. |
| 英文摘要 | The objective of this study was to develop type I markers for genome mapping and other genetic studies of Penaeus monodon. Primers were designed based on expressed sequence tags (ESTs) from a P monodon cephalothorax cDNA library to amplify 100-300 bp products. 34 of the primer pairs successfully amplified PCR products from genomic DNA. Single-strand conformation polymorphism analysis showed that similar to 30% of the ESTs tested exhibit polymorphism in a test panel of P monodon individuals. Mendelian inheritance of the EST-derived markers has been established in two international reference mapping families of P monodon, and mapping of these markers is in progress. Some ESTs were successfully amplified from other Penaeus species (P. chinensis, P japonicus and P vannamei), indicating that the markers are applicable in cross-species comparison. Two populations of P. japonicus could be differentiated using one of the ESTS. In conclusion, the polymorphic EST markers developed in this study are applicable in genome mapping and population genetic studies of penaeid shrimp. (C) 2002 Elsevier Science B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Marine & Freshwater Biology |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology |
| 关键词[WOS] | EXPRESSED SEQUENCE TAGS ; BLACK TIGER PRAWN ; SHRIMP PENAEUS ; MICROSATELLITE MARKERS ; POPULATION-STRUCTURE ; KURUMA PRAWN ; JAPONICUS ; PACIFIC ; GENOME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000176129000007 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9842]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Univ Hong Kong, Dept Biol, Shatin, Hong Kong, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 3.CSIRO, Mol Anim Genet ctr, Livestock Ind, St Lucia, Qld 4072, Australia |
| 推荐引用方式 GB/T 7714 | Tong, J,Lehnert, SA,Byrne, K,et al. Development of polymorphic EST markers in Penaeus monodon: applications in penaeid genetics[J]. AQUACULTURE,2002,208(1-2):69-79. |
| APA | Tong, J,Lehnert, SA,Byrne, K,Kwan, HS,&Chu, KH.(2002).Development of polymorphic EST markers in Penaeus monodon: applications in penaeid genetics.AQUACULTURE,208(1-2),69-79. |
| MLA | Tong, J,et al."Development of polymorphic EST markers in Penaeus monodon: applications in penaeid genetics".AQUACULTURE 208.1-2(2002):69-79. |
入库方式： OAI收割
来源：水生生物研究所
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