Phylogenetic relationships among six species of Epistylis inferred from 18S-ITS1 sequences
文献类型：期刊论文
| 作者 | Miao, W; Yu, YH; Shen, YF; Zhang, XY |
| 刊名 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES
 |
| 出版日期 | 2002-06-01 |
| 卷号 | 45期号:3页码:280-288 |
| 关键词 | Epistylis 18S-ITS1 region phylogenetic relationships method in situ (prepare DNA from field-sampled species for PCR) macronucleus |
| ISSN号 | 1006-9305 |
| 通讯作者 | Shen, YF, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | Phylogenetic relationships among six species of Epistylis (i.e. E. plicatilis, E. urceolata, E. chrysemydis, E. hentscheli, E. wenrichi, and E, galea) were investigated using sequences of the first internal transcribed spacer region (ITS-1) of ribosomal DNA (rDNA). Amplified rDNA fragment sequences consisted of 215 or 217 bases of the flanking 18S and 5.8S regions, and the entire ITS-1 region (from 145 to 155 bases). There were more than 33 variable bases between E. galea and the other five species in both the 18S region and the ITS-1 region. The affiliation of them was assessed using Neighbor-joining (NJ), maximum parsimony (MP) and maximum likelihood (ML) analyses. In all the NJ, MP and ML analyses E. galea, whose macronucleic position and shape are distinctly different from those of the other five species, was probably diverged from the ancestor of Epistylis earlier than the other five species. The topology in which E. plicatilis and E, hentscheli formed a strongly supported sister clade to E. urceolata, E. chrysemydis, and E. wenrichi was consistent with variations in the thickness of the peristomial lip. We concluded that the macronucleus and peristomial lip might be the important phylogenetic characteristics within the genus Epistylis. |
| 英文摘要 | Phylogenetic relationships among six species of Epistylis (i.e. E. plicatilis, E. urceolata, E. chrysemydis, E. hentscheli, E. wenrichi, and E, galea) were investigated using sequences of the first internal transcribed spacer region (ITS-1) of ribosomal DNA (rDNA). Amplified rDNA fragment sequences consisted of 215 or 217 bases of the flanking 18S and 5.8S regions, and the entire ITS-1 region (from 145 to 155 bases). There were more than 33 variable bases between E. galea and the other five species in both the 18S region and the ITS-1 region. The affiliation of them was assessed using Neighbor-joining (NJ), maximum parsimony (MP) and maximum likelihood (ML) analyses. In all the NJ, MP and ML analyses E. galea, whose macronucleic position and shape are distinctly different from those of the other five species, was probably diverged from the ancestor of Epistylis earlier than the other five species. The topology in which E. plicatilis and E, hentscheli formed a strongly supported sister clade to E. urceolata, E. chrysemydis, and E. wenrichi was consistent with variations in the thickness of the peristomial lip. We concluded that the macronucleus and peristomial lip might be the important phylogenetic characteristics within the genus Epistylis. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biology |
| 类目[WOS] | Biology |
| 研究领域[WOS] | Life Sciences & Biomedicine - Other Topics |
| 关键词[WOS] | SUBUNIT ; EVOLUTION ; GENES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000175701400007 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9862]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Miao, W,Yu, YH,Shen, YF,et al. Phylogenetic relationships among six species of Epistylis inferred from 18S-ITS1 sequences[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2002,45(3):280-288. |
| APA | Miao, W,Yu, YH,Shen, YF,&Zhang, XY.(2002).Phylogenetic relationships among six species of Epistylis inferred from 18S-ITS1 sequences.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,45(3),280-288. |
| MLA | Miao, W,et al."Phylogenetic relationships among six species of Epistylis inferred from 18S-ITS1 sequences".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 45.3(2002):280-288. |
入库方式： OAI收割
来源：水生生物研究所
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