Three-piece-ligation PCR and application in disruption of chlorophyll synthesis genes in Synechocystis sp PCC 6803
文献类型：期刊论文
| 作者 | Kong, RQ; Xu, XD |
| 刊名 | CURRENT MICROBIOLOGY
 |
| 出版日期 | 2002-04-01 |
| 卷号 | 44期号:4页码:241-245 |
| 关键词 | ESCHERICHIA-COLI BIOSYNTHESIS PCC-6803 PCC6803 |
| ISSN号 | 0343-8651 |
| 通讯作者 | Xu, XD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China |
| 中文摘要 | Linear DNA, consisting of a drug-resistance marker and long flanking sequences, was synthesized by one-step polymerase chain reaction after a three-piece ligating reaction. Chlorophyll synthesis genes, chlH and chIL in Synechocystis sp. PCC 6803, were replaced by a kanamycin-resistance marker through double recombinations with flanking homology regions. Under LAHG conditions, the chIL but not chlH mutant stopped chlorophyll synthesis, while both synthesized chlorophyll in the light. |
| 英文摘要 | Linear DNA, consisting of a drug-resistance marker and long flanking sequences, was synthesized by one-step polymerase chain reaction after a three-piece ligating reaction. Chlorophyll synthesis genes, chlH and chIL in Synechocystis sp. PCC 6803, were replaced by a kanamycin-resistance marker through double recombinations with flanking homology regions. Under LAHG conditions, the chIL but not chlH mutant stopped chlorophyll synthesis, while both synthesized chlorophyll in the light. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Microbiology |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | ESCHERICHIA-COLI ; BIOSYNTHESIS ; PCC-6803 ; PCC6803 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000174316400003 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9876]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kong, RQ,Xu, XD. Three-piece-ligation PCR and application in disruption of chlorophyll synthesis genes in Synechocystis sp PCC 6803[J]. CURRENT MICROBIOLOGY,2002,44(4):241-245. |
| APA | Kong, RQ,&Xu, XD.(2002).Three-piece-ligation PCR and application in disruption of chlorophyll synthesis genes in Synechocystis sp PCC 6803.CURRENT MICROBIOLOGY,44(4),241-245. |
| MLA | Kong, RQ,et al."Three-piece-ligation PCR and application in disruption of chlorophyll synthesis genes in Synechocystis sp PCC 6803".CURRENT MICROBIOLOGY 44.4(2002):241-245. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。