热门
Characterization of an iridovirus from the cultured pig frog Rana grylio with lethal syndrome
文献类型：期刊论文
| 作者 | Zhang, QY; Xiao, F; Li, ZQ; Gui, JF; Mao, JH; Chinchar, VG |
| 刊名 | DISEASES OF AQUATIC ORGANISMS
 |
| 出版日期 | 2001-12-20 |
| 卷号 | 48期号:1页码:27-36 |
| ISSN号 | 0177-5103 |
| 关键词 | Rana grylio iridovirus FV3 Ranavirus viral disease |
| 通讯作者 | Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | Three virus isolates, RGV-9506, RGV-9807 and RGV-9808, were obtained from cultured pig frogs Rana grylio undergoing lethal infections. Previously, the first isolate, RGV-9506, was shown to be an iridovirus based on ultrastructural and morphological studies. In the present study, the original isolate, along with 2 recent ones, were more extensively characterized by experimental infection studies, histopathology, electron microscopy, serological reactivity, gel electrophoresis of viral polypeptides and DNA restriction fragments, PCR amplification, and nucleic acid sequence analysis of the major capsid protein (MCP) gene. The 3 isolates were shown to be identical to each other, and very similar to FV3, the type species of the genus Ranavirus (family Iridoviridae). These results suggest that RGV should be considered a strain of FV3, and indicate that FV3-like iridoviruses are capable of causing widespread, severe disease among cultured frogs. |
| 英文摘要 | Three virus isolates, RGV-9506, RGV-9807 and RGV-9808, were obtained from cultured pig frogs Rana grylio undergoing lethal infections. Previously, the first isolate, RGV-9506, was shown to be an iridovirus based on ultrastructural and morphological studies. In the present study, the original isolate, along with 2 recent ones, were more extensively characterized by experimental infection studies, histopathology, electron microscopy, serological reactivity, gel electrophoresis of viral polypeptides and DNA restriction fragments, PCR amplification, and nucleic acid sequence analysis of the major capsid protein (MCP) gene. The 3 isolates were shown to be identical to each other, and very similar to FV3, the type species of the genus Ranavirus (family Iridoviridae). These results suggest that RGV should be considered a strain of FV3, and indicate that FV3-like iridoviruses are capable of causing widespread, severe disease among cultured frogs. |
| 学科主题 | Fisheries; Veterinary Sciences |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Veterinary Sciences |
| 关键词[WOS] | EPIZOOTIC HEMATOPOIETIC NECROSIS ; CHAIN-REACTION PCR ; MOLECULAR CHARACTERIZATION ; BOHLE IRIDOVIRUS ; RAINBOW-TROUT ; BUFO-MARINUS ; FISH ; VIRUS ; AUSTRALIA ; SEQUENCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000174168000004 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9878]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Univ Mississippi, Med Ctr, Dept Microbiol, Jackson, MS 39216 USA |
| 推荐引用方式 GB/T 7714 | Zhang, QY,Xiao, F,Li, ZQ,et al. Characterization of an iridovirus from the cultured pig frog Rana grylio with lethal syndrome[J]. DISEASES OF AQUATIC ORGANISMS,2001,48(1):27-36. |
| APA | Zhang, QY,Xiao, F,Li, ZQ,Gui, JF,Mao, JH,&Chinchar, VG.(2001).Characterization of an iridovirus from the cultured pig frog Rana grylio with lethal syndrome.DISEASES OF AQUATIC ORGANISMS,48(1),27-36. |
| MLA | Zhang, QY,et al."Characterization of an iridovirus from the cultured pig frog Rana grylio with lethal syndrome".DISEASES OF AQUATIC ORGANISMS 48.1(2001):27-36. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。