In vitro culture of embryonic hearts from guppy fish (Poecilia reticulata)
文献类型：期刊论文
| 作者 | Zhang, QY; Li, ZQ; Gui, JF |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2001-10-01 |
| 卷号 | 46期号:19页码:1638-+ |
| ISSN号 | 1001-6538 |
| 关键词 | guppy fish embryo heart in vitro culture |
| 通讯作者 | Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Forty embryonic hearts were taken out by anatomical needle from denuded embryos of the ovoviviparity guppy fish that were dechorioned by mechanic method or by trypsin digestion, and were in vitro cultured. In the cultured hearts, 80% have maintained beating in vitro for 4 weeks, and the longest record for beating was 142 d. Owing to fish embryo transparency, beating frequency and blood color changes are easily viewed from the embryonic hearts under a dissecting microscope. The current study established the in vitro culture method of embryonic hearts in guppy fish, which can be used as a model for the study of heart and cardiovascular system in vertebrates. |
| 英文摘要 | Forty embryonic hearts were taken out by anatomical needle from denuded embryos of the ovoviviparity guppy fish that were dechorioned by mechanic method or by trypsin digestion, and were in vitro cultured. In the cultured hearts, 80% have maintained beating in vitro for 4 weeks, and the longest record for beating was 142 d. Owing to fish embryo transparency, beating frequency and blood color changes are easily viewed from the embryonic hearts under a dissecting microscope. The current study established the in vitro culture method of embryonic hearts in guppy fish, which can be used as a model for the study of heart and cardiovascular system in vertebrates. |
| 学科主题 | Multidisciplinary Sciences |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | IN-VITRO ; STEM-CELLS ; HEMATOPOIESIS ; MORPHOGENESIS ; SYSTEM ; MODEL ; VIRUS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000172324000016 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9918]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, QY,Li, ZQ,Gui, JF. In vitro culture of embryonic hearts from guppy fish (Poecilia reticulata)[J]. CHINESE SCIENCE BULLETIN,2001,46(19):1638-+. |
| APA | Zhang, QY,Li, ZQ,&Gui, JF.(2001).In vitro culture of embryonic hearts from guppy fish (Poecilia reticulata).CHINESE SCIENCE BULLETIN,46(19),1638-+. |
| MLA | Zhang, QY,et al."In vitro culture of embryonic hearts from guppy fish (Poecilia reticulata)".CHINESE SCIENCE BULLETIN 46.19(2001):1638-+. |
入库方式： OAI收割
来源：水生生物研究所
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