Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis
文献类型：期刊论文
| 作者 | Wen, JJ; Xie, J; Liu, SG; Gui, JF |
| 刊名 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES
 |
| 出版日期 | 2001-10-01 |
| 卷号 | 44期号:5页码:541-553 |
| ISSN号 | 1006-9305 |
| 关键词 | silver crucian carp oocyte oogenesis suppressive subtractive hybridization WD domain |
| 通讯作者 | Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | A new gene with WD domains is cloned and characterized according to its differential transcription and expression between previtellogenic oocytes (phase I oocytes) and fully-grown oocytes (phase V oocytes) from natural gynogenetic silver crucian carp (Carassius auratus gibelio) by using the combinative methods of suppressive subtraction hybridization, SMART cDNA synthesis and RACE-PCR. The full-length cDNA is 1870 bp. Its 5 ' untranslated region is 210 bp, followed by an open reading frame of 990 bp, which has the typical vertebrate initiator codon of ANNATG. The open reading frame encodes a protein with 329 amino acids. It has 670 bp of 3 ' untranslated region and an AATAAA polyadenylation signal. Because it has 92% homology to STRAP (serine-threonine kinase receptor-associated protein), a recently reported gene, we named it FSTRAP (fish STRAP). Virtual Northern blotting indicated that the FSTRAP was transcribed in fully-grown oocytes (phase V oocytes), but not in previtellogenic oocytes (phase I oocytes). RT-PCR analysis showed that FSTRAP was transcribed in brain, heart, kidney, muscle, ovary, spleen and testis, but not in liver. And its mRNA could be detected in the oocytes from phase II to phase V. Western blotting also showed that FSTRAP protein could be detected in brain, heart, kidney, muscle, ovary, spleen and testis except liver. Results of Western blotting on various oocytes were also similar to the RT-PCR data. FSTRAP protein was not expressed in the previtellogenic oocytes. Its expression initiated from phase II oocytes after vitellogenesis, and was consistent with the mRNA transcription. |
| 英文摘要 | A new gene with WD domains is cloned and characterized according to its differential transcription and expression between previtellogenic oocytes (phase I oocytes) and fully-grown oocytes (phase V oocytes) from natural gynogenetic silver crucian carp (Carassius auratus gibelio) by using the combinative methods of suppressive subtraction hybridization, SMART cDNA synthesis and RACE-PCR. The full-length cDNA is 1870 bp. Its 5 ' untranslated region is 210 bp, followed by an open reading frame of 990 bp, which has the typical vertebrate initiator codon of ANNATG. The open reading frame encodes a protein with 329 amino acids. It has 670 bp of 3 ' untranslated region and an AATAAA polyadenylation signal. Because it has 92% homology to STRAP (serine-threonine kinase receptor-associated protein), a recently reported gene, we named it FSTRAP (fish STRAP). Virtual Northern blotting indicated that the FSTRAP was transcribed in fully-grown oocytes (phase V oocytes), but not in previtellogenic oocytes (phase I oocytes). RT-PCR analysis showed that FSTRAP was transcribed in brain, heart, kidney, muscle, ovary, spleen and testis, but not in liver. And its mRNA could be detected in the oocytes from phase II to phase V. Western blotting also showed that FSTRAP protein could be detected in brain, heart, kidney, muscle, ovary, spleen and testis except liver. Results of Western blotting on various oocytes were also similar to the RT-PCR data. FSTRAP protein was not expressed in the previtellogenic oocytes. Its expression initiated from phase II oocytes after vitellogenesis, and was consistent with the mRNA transcription. |
| 学科主题 | Biology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biology |
| 研究领域[WOS] | Life Sciences & Biomedicine - Other Topics |
| 关键词[WOS] | SILVER CRUCIAN CARP ; GROWTH-FACTOR-BETA ; TROUT ONCORHYNCHUS-MYKISS ; XENOPUS-LAEVIS OOCYTES ; AURATUS GIBELIO BLOCH ; I IGF-I ; RAINBOW-TROUT ; INVOLVEMENT ; SUPPRESSION ; RECEPTORS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000171966600011 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9920]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wen, JJ,Xie, J,Liu, SG,et al. Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2001,44(5):541-553. |
| APA | Wen, JJ,Xie, J,Liu, SG,&Gui, JF.(2001).Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,44(5),541-553. |
| MLA | Wen, JJ,et al."Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 44.5(2001):541-553. |
入库方式： OAI收割
来源：水生生物研究所
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