Genome segment S8 of grass carp hemorrhage virus encodes a virion protein
文献类型：期刊论文
| 作者 | Qiu, T; Zhang, J; Lu, RH; Zhu, ZY |
| 刊名 | INTERVIROLOGY
 |
| 出版日期 | 2001-09-01 |
| 卷号 | 44期号:5页码:317-320 |
| ISSN号 | 0300-5526 |
| 关键词 | RNA virus GCHV segment S8 nucleoticle sequence virion protein IgY |
| 通讯作者 | Lu, RH, Chinese Acad Sci, Inst Hydrobiol, IHB,State Key Lab Freshwater Ecol & Biotechnol, Dept Genet & Breeding, Wuhan 430072, Peoples R China |
| 中文摘要 | The complete nucleotide sequence of the genome segment S8 of grass carp hemorrhage virus (GCHV) was determined from cDNA corresponding to the viral genomic RNA. It is 1,287 nucleotides in length and contains a large open reading frame that could encode a protein of 409 amino acids with a predicted molecular mass of 44 kD. The S8 was expressed using the pET fusion protein vector and detected by Western blotting analysis using the chicken egg IgY against intact GCHV particles, indicating that S8 encodes a virion protein. Amino acid sequence comparisons revealed that the protein encoded by S8 is closely related to protein alpha2 of mammalian reovirus, suggesting that the deduced protein of S8 is an inner capsid protein. Copyright (C) 2001 S. Karger AG, Basel. |
| 英文摘要 | The complete nucleotide sequence of the genome segment S8 of grass carp hemorrhage virus (GCHV) was determined from cDNA corresponding to the viral genomic RNA. It is 1,287 nucleotides in length and contains a large open reading frame that could encode a protein of 409 amino acids with a predicted molecular mass of 44 kD. The S8 was expressed using the pET fusion protein vector and detected by Western blotting analysis using the chicken egg IgY against intact GCHV particles, indicating that S8 encodes a virion protein. Amino acid sequence comparisons revealed that the protein encoded by S8 is closely related to protein alpha2 of mammalian reovirus, suggesting that the deduced protein of S8 is an inner capsid protein. Copyright (C) 2001 S. Karger AG, Basel. |
| 学科主题 | Virology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Virology |
| 研究领域[WOS] | Virology |
| 关键词[WOS] | SEQUENCE ; REOVIRUS ; AQUAREOVIRUS ; CLONING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000171984100007 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9922]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, IHB,State Key Lab Freshwater Ecol & Biotechnol, Dept Genet & Breeding, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu, T,Zhang, J,Lu, RH,et al. Genome segment S8 of grass carp hemorrhage virus encodes a virion protein[J]. INTERVIROLOGY,2001,44(5):317-320. |
| APA | Qiu, T,Zhang, J,Lu, RH,&Zhu, ZY.(2001).Genome segment S8 of grass carp hemorrhage virus encodes a virion protein.INTERVIROLOGY,44(5),317-320. |
| MLA | Qiu, T,et al."Genome segment S8 of grass carp hemorrhage virus encodes a virion protein".INTERVIROLOGY 44.5(2001):317-320. |
入库方式： OAI收割
来源：水生生物研究所
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