Phylogenetic relationships of the subclass Peritrichia (Oligohymenophorea, Ciliophora) with emphasis on the genus Epistylis, inferred from small subunit rRNA gene sequences
文献类型：期刊论文
| 作者 | Miao, W; Yu, YH; Shen, YF |
| 刊名 | JOURNAL OF EUKARYOTIC MICROBIOLOGY
 |
| 出版日期 | 2001-09-01 |
| 卷号 | 48期号:5页码:583-587 |
| ISSN号 | 1066-5234 |
| 关键词 | field-sampled species for PCR monophyly peristomial lip Vorticella |
| 通讯作者 | Shen, YF, Chinese Acad Sci, Inst Hydrobiol, Lab Protozoan Taxon & Ecol, Wuhan 430072, Peoples R China |
| 中文摘要 | The peritrichs have been recognized as a higher taxon of ciliates since 1968. However, the phylogenetic relationships among them are still unsettled, and their placement within the class Oligohymenophorea has only been supported by the analysis of the small subunit rRNA gene sequence of Opisthonecta henneguyi. DNA was isolated directly from field-sampled species for PCR, and was used to resolve relationships within the genus Epistylis and to confirm the stability of the placement of peritrichs. Small subunit rRNA gene sequences of Epistylis plicatilis, Epistylis urceolata, Epistylis chysemydis, Epistylis hentscheli, Epistylis wenrichi, and Vorticella campanula were sequenced and analyzed using both distance-matrix and maximum-parsimony methods. In phylogenetic trees, the monophyly of both the genus Epistylis and the subclass Peritrichia was strongly supported, while V. campanula clustered with Vorticella microstoma. The topology in which E. plicatilis and E. hentscheli formed a strongly supported sister clade to E. urceolata, E. chrysemydis, and E. wenrichi was consistent with variations in the thickness of the peristomial lip. We concluded that the peristomial area, especially the. peristomial lip, might be the important phylogenetic character within the genus Epistylis. |
| 英文摘要 | The peritrichs have been recognized as a higher taxon of ciliates since 1968. However, the phylogenetic relationships among them are still unsettled, and their placement within the class Oligohymenophorea has only been supported by the analysis of the small subunit rRNA gene sequence of Opisthonecta henneguyi. DNA was isolated directly from field-sampled species for PCR, and was used to resolve relationships within the genus Epistylis and to confirm the stability of the placement of peritrichs. Small subunit rRNA gene sequences of Epistylis plicatilis, Epistylis urceolata, Epistylis chysemydis, Epistylis hentscheli, Epistylis wenrichi, and Vorticella campanula were sequenced and analyzed using both distance-matrix and maximum-parsimony methods. In phylogenetic trees, the monophyly of both the genus Epistylis and the subclass Peritrichia was strongly supported, while V. campanula clustered with Vorticella microstoma. The topology in which E. plicatilis and E. hentscheli formed a strongly supported sister clade to E. urceolata, E. chrysemydis, and E. wenrichi was consistent with variations in the thickness of the peristomial lip. We concluded that the peristomial area, especially the. peristomial lip, might be the important phylogenetic character within the genus Epistylis. |
| 学科主题 | Microbiology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | RIBOSOMAL-RNA SEQUENCES ; TETRAHYMENA CILIOPHORA ; PHYLUM CILIOPHORA ; EVOLUTION ; INSIGHTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000171320700009 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9934]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, Lab Protozoan Taxon & Ecol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Miao, W,Yu, YH,Shen, YF. Phylogenetic relationships of the subclass Peritrichia (Oligohymenophorea, Ciliophora) with emphasis on the genus Epistylis, inferred from small subunit rRNA gene sequences[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2001,48(5):583-587. |
| APA | Miao, W,Yu, YH,&Shen, YF.(2001).Phylogenetic relationships of the subclass Peritrichia (Oligohymenophorea, Ciliophora) with emphasis on the genus Epistylis, inferred from small subunit rRNA gene sequences.JOURNAL OF EUKARYOTIC MICROBIOLOGY,48(5),583-587. |
| MLA | Miao, W,et al."Phylogenetic relationships of the subclass Peritrichia (Oligohymenophorea, Ciliophora) with emphasis on the genus Epistylis, inferred from small subunit rRNA gene sequences".JOURNAL OF EUKARYOTIC MICROBIOLOGY 48.5(2001):583-587. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。