Differential gene expression in fully-grown oocytes between gynogenetic and gonochoristic crucian carps
文献类型:期刊论文
作者 | Xie, J; Wen, JJ; Chen, B; Gui, JF |
刊名 | GENE |
出版日期 | 2001-06-13 |
卷号 | 271期号:1页码:109-116 |
ISSN号 | 0378-1119 |
关键词 | differential gene expression gynogenesis oogenesis oocytes suppression subtractive hybridization |
通讯作者 | Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
中文摘要 | Silver crucian carp (Carassius auratus gibelio) is a unique triploid bisexual species that can reproduce by gynogenesis. As all other gynogenetic animals, it keeps its chromosome integrity by inhibiting the first meiosis division (no extrusion of the first pole body). To understand the molecular events governing this reproduction mode, suppression subtractive hybridization was used to identify the genes differentially expressed in fully-grown oocytes of the gynogenetic and gonochoristic crucian carp (gyno-carp and gono-carp). From two specific subtractive cDNA libraries, the clones screened out by dot blots and virtual Northern blots were chosen to clone, full-length cDNA by RACE. Four differentially expressed genes were obtained. Two are novel genes and are expressed specifically in the oocytes. The gyno-carp stores much more mRNA of cyclin A2, a new member of the fish A-type cyclin gene, in its fully-grown oocyte than in the gono-carp. The last gene is histone H2A. The histone H2As of these two closely related crucian carps are quite different in the C-terminus. Preliminary characterization of the four genes has been analyzed by nucleotide and deduced amino acid sequence and Northern analysis. (C) 2001 Elsevier Science B.V. All rights reserved. |
英文摘要 | Silver crucian carp (Carassius auratus gibelio) is a unique triploid bisexual species that can reproduce by gynogenesis. As all other gynogenetic animals, it keeps its chromosome integrity by inhibiting the first meiosis division (no extrusion of the first pole body). To understand the molecular events governing this reproduction mode, suppression subtractive hybridization was used to identify the genes differentially expressed in fully-grown oocytes of the gynogenetic and gonochoristic crucian carp (gyno-carp and gono-carp). From two specific subtractive cDNA libraries, the clones screened out by dot blots and virtual Northern blots were chosen to clone, full-length cDNA by RACE. Four differentially expressed genes were obtained. Two are novel genes and are expressed specifically in the oocytes. The gyno-carp stores much more mRNA of cyclin A2, a new member of the fish A-type cyclin gene, in its fully-grown oocyte than in the gono-carp. The last gene is histone H2A. The histone H2As of these two closely related crucian carps are quite different in the C-terminus. Preliminary characterization of the four genes has been analyzed by nucleotide and deduced amino acid sequence and Northern analysis. (C) 2001 Elsevier Science B.V. All rights reserved. |
学科主题 | Genetics & Heredity |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Genetics & Heredity |
研究领域[WOS] | Genetics & Heredity |
关键词[WOS] | CARASSIUS-AURATUS-LANGSDORFII ; GIBELIO BLOCH ; CELL CYCLE ; ARREST ; MATURATION ; CHROMATIN ; DISCOVERY ; KINASE ; FISH |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000170329200013 |
公开日期 | 2010-10-13 |
源URL | [http://ir.ihb.ac.cn/handle/152342/9956] |
专题 | 水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文 |
作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
推荐引用方式 GB/T 7714 | Xie, J,Wen, JJ,Chen, B,et al. Differential gene expression in fully-grown oocytes between gynogenetic and gonochoristic crucian carps[J]. GENE,2001,271(1):109-116. |
APA | Xie, J,Wen, JJ,Chen, B,&Gui, JF.(2001).Differential gene expression in fully-grown oocytes between gynogenetic and gonochoristic crucian carps.GENE,271(1),109-116. |
MLA | Xie, J,et al."Differential gene expression in fully-grown oocytes between gynogenetic and gonochoristic crucian carps".GENE 271.1(2001):109-116. |
入库方式: OAI收割
来源:水生生物研究所
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