Dendritic Poly(L-lysine)-b-Poly(L-lactide)-b-Dendritic Poly(L-lysine) Amphiphilic Gene Delivery Vectors: Roles of PLL Dendritic Generation and Enhanced Transgene Efficacies via Termini Modification
文献类型：期刊论文
| 作者 | Li Y(李洋) ; Zhu YD(朱英丹) ; Xia KJ(夏可嘉) ; Sheng RL(盛瑞隆) ; Jia L(贾林) ; Hou XD(侯小东) ; Xu YH(徐宇虹) ; Cao AM(曹阿民) |
| 刊名 | Biomacromolecules
 |
| 出版日期 | 2009 |
| 卷号 | 10期号:8页码:2284-2293 |
| ISSN号 | 1525-7797 |
| 其他题名 | 聚乳酸-树枝状聚赖氨酸两亲基因载体的研究：赖氨酸代数的作用以及端基修饰增强转基因效率 |
| 通讯作者 | 徐宇虹 ; 曹阿民 |
| 英文摘要 | As an effort to prepare new efficient gene delivery vectors, we have recently developed and reported an amphiphilic dendritic poly(L-lysine)-b-poly(L-lactide)-b-dendritic poly(L-lysine) D-2-PLLA-D-2 with two-generation PLL dendrons and a PLLA block. In this work, we continued to explore the roles of dendritic PLL generation in DNA binding and intracellular delivery of gene, and a new series of amphiphilic dendritic poly(L-lysine)-b-poly(L-lactide)-b-dendritic poly(L-lysine)s D-n-PLLA-D-n (n = 3-5) were synthesized and were structurally characterized. Furthermore, plasmid DNA binding affinity for these cationic amphiphiles was examined by agarose gel electrophoresis and fluorescence titration assay in pure water and PBS buffer solution containing 150 mM NaCl (pH = 7.4), respectively. By dynamic light scattering (DLS) and transmission electronic microscopy (TEM), the interaction and complexation in between were investigated, concerning the DNA/vector polyplex particle morphologies and zeta potentials. Utilizing a human hepatocellular carcinoma cell-line SMMC-7721, cell toxicity, and gene transfection in vitro were explored. To further improve transgene efficiency for these synthetic cationic amphiphiles as gene delivery vectors, new structural DEn-PLLA-DEn (n = 2-3) were prepared through an amino termini modification of the D-n-PLLA-D-n (n = 2-3) with less toxic 4,7,10,13-tetraazatridecanoic acids, and gene transfection with these DEn-PLLA-DEn (n = 2-3) was examined with an alternative human gastric carcinoma cell-line HGC-27. As a result, the high plasmid DNA binding affinity, low cytotoxicity, and much enhanced transgene efficacy suggest a new possible clue to design effective synthetic gene delivery vectors with amphiphilic skeleton and less toxic polyamine building blocks. |
| 学科主题 | 高分子化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1021/bm900456x |
| 语种 | 英语 |
| WOS记录号 | WOS:000268661400034 |
| 公开日期 | 2013-03-07 |
| 源URL | [http://202.127.28.38/handle/331003/22940]  |
| 专题 | 上海有机化学研究所_高分子材料研究室 |
| 推荐引用方式 GB/T 7714 | Li Y,Zhu YD,Xia KJ,et al. Dendritic Poly(L-lysine)-b-Poly(L-lactide)-b-Dendritic Poly(L-lysine) Amphiphilic Gene Delivery Vectors: Roles of PLL Dendritic Generation and Enhanced Transgene Efficacies via Termini Modification[J]. Biomacromolecules,2009,10(8):2284-2293. |
| APA | 李洋.,朱英丹.,夏可嘉.,盛瑞隆.,贾林.,...&曹阿民.(2009).Dendritic Poly(L-lysine)-b-Poly(L-lactide)-b-Dendritic Poly(L-lysine) Amphiphilic Gene Delivery Vectors: Roles of PLL Dendritic Generation and Enhanced Transgene Efficacies via Termini Modification.Biomacromolecules,10(8),2284-2293. |
| MLA | 李洋,et al."Dendritic Poly(L-lysine)-b-Poly(L-lactide)-b-Dendritic Poly(L-lysine) Amphiphilic Gene Delivery Vectors: Roles of PLL Dendritic Generation and Enhanced Transgene Efficacies via Termini Modification".Biomacromolecules 10.8(2009):2284-2293. |
入库方式： OAI收割
来源：上海有机化学研究所
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