Molecular characterization and expression of the M6 gene of grass carp hemorrhage virus (GCHV), an aquareovirus - Brief report
文献类型：期刊论文
| 作者 | Qiu, T; Lu, RH; Zhang, J; Zhu, ZY |
| 刊名 | ARCHIVES OF VIROLOGY
 |
| 出版日期 | 2001 |
| 卷号 | 146期号:7页码:1391-1397 |
| ISSN号 | 0304-8608 |
| 关键词 | RNA BLOT HYBRIDIZATION GENOME IDENTIFICATION GENOGROUP SEQUENCE SEGMENTS MU-1C |
| 通讯作者 | Lu, RH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | 7The complete nucleotide sequence of M6 gene of grass carp hemorrhage virus (GCHV) was determined. It is 2039 nucleotides in length and contains a single large open reading frame that could encode a protein of 648 amino acids with predicted molecular mass of 68.7 kDa. Amino acid sequence comparison revealed that the protein encoded by GCHV M6 is closely related to the protein mul of mammalian reovirus. The M6 gene, encoding the major outer-capsid protein, was expressed using the pET fusion protein vector in Escherichia coli and detected by Western blotting using chicken anti-GCHV immunoglobulin (IgY). The result indicates that the protein encoded by M6 may share a putative Asn-42-Pro-43 proteolytic cleavage site with mul. |
| 英文摘要 | 7The complete nucleotide sequence of M6 gene of grass carp hemorrhage virus (GCHV) was determined. It is 2039 nucleotides in length and contains a single large open reading frame that could encode a protein of 648 amino acids with predicted molecular mass of 68.7 kDa. Amino acid sequence comparison revealed that the protein encoded by GCHV M6 is closely related to the protein mul of mammalian reovirus. The M6 gene, encoding the major outer-capsid protein, was expressed using the pET fusion protein vector in Escherichia coli and detected by Western blotting using chicken anti-GCHV immunoglobulin (IgY). The result indicates that the protein encoded by M6 may share a putative Asn-42-Pro-43 proteolytic cleavage site with mul. |
| 学科主题 | Virology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Virology |
| 研究领域[WOS] | Virology |
| 关键词[WOS] | RNA BLOT HYBRIDIZATION ; GENOME ; IDENTIFICATION ; GENOGROUP ; SEQUENCE ; SEGMENTS ; MU-1C |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000170368200012 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9966]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu, T,Lu, RH,Zhang, J,et al. Molecular characterization and expression of the M6 gene of grass carp hemorrhage virus (GCHV), an aquareovirus - Brief report[J]. ARCHIVES OF VIROLOGY,2001,146(7):1391-1397. |
| APA | Qiu, T,Lu, RH,Zhang, J,&Zhu, ZY.(2001).Molecular characterization and expression of the M6 gene of grass carp hemorrhage virus (GCHV), an aquareovirus - Brief report.ARCHIVES OF VIROLOGY,146(7),1391-1397. |
| MLA | Qiu, T,et al."Molecular characterization and expression of the M6 gene of grass carp hemorrhage virus (GCHV), an aquareovirus - Brief report".ARCHIVES OF VIROLOGY 146.7(2001):1391-1397. |
入库方式： OAI收割
来源：水生生物研究所
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