Isolation by phage display and characterization of a single-chain antibody specific for O-6-methyldeoxyguanosine
文献类型：期刊论文
| 作者 | Dai, HP; Zhao, RH; Brenda, H; Hemmingsen, SM; Xiao, W |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2001-06-01 |
| 卷号 | 46期号:12页码:1024-1029 |
| 关键词 | O-6-methyl-2 '-deoxyguanosine phage display antibody single chain Fv DNA adducts |
| ISSN号 | 1001-6538 |
| 通讯作者 | Hemmingsen, SM, Natl Res Council Canada, Inst Plant Biotechnol, 110 Gymnasium Rd, Saskatoon, SK S7N 0W9, Canada |
| 中文摘要 | New approaches of making single chain Fv antibodies against O-6-methyl-2'-deoxyguanosine (O(6)MdG) have been demonstrated by using the phage antibody display system. Using O(6)MdG as an antigen, 21 positive clones were identified by ELISA from this library, one of which, designated H3, specifically binds to O(6)MdG with high affinity. The H3 scFv antibody has an affinity constant (K-aff) of 5.94 x 10(11)(mol/L)(-1). H3 scFv has been successfully used to detect O-6 MdG in DNA hydrolyses from yeast or E. coli cells treated with a DNA methylating agent. To our knowledge, this is the first report of the selection of a specific scFv against DNA adducts. The results demonstrate the potential applications of the phage display technology for the detection of DNA lesions caused by mutagens and carcinogens. |
| 英文摘要 | New approaches of making single chain Fv antibodies against O-6-methyl-2'-deoxyguanosine (O(6)MdG) have been demonstrated by using the phage antibody display system. Using O(6)MdG as an antigen, 21 positive clones were identified by ELISA from this library, one of which, designated H3, specifically binds to O(6)MdG with high affinity. The H3 scFv antibody has an affinity constant (K-aff) of 5.94 x 10(11)(mol/L)(-1). H3 scFv has been successfully used to detect O-6 MdG in DNA hydrolyses from yeast or E. coli cells treated with a DNA methylating agent. To our knowledge, this is the first report of the selection of a specific scFv against DNA adducts. The results demonstrate the potential applications of the phage display technology for the detection of DNA lesions caused by mutagens and carcinogens. |
| WOS标题词 | Science & Technology |
| 学科主题 | Multidisciplinary Sciences |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | ESCHERICHIA-COLI ; DNA ; REPAIR ; O-6-METHYLGUANINE ; O6-METHYLGUANINE ; YEAST |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000170193900014 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9968]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 3.Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada |
| 推荐引用方式 GB/T 7714 | Dai, HP,Zhao, RH,Brenda, H,et al. Isolation by phage display and characterization of a single-chain antibody specific for O-6-methyldeoxyguanosine[J]. CHINESE SCIENCE BULLETIN,2001,46(12):1024-1029. |
| APA | Dai, HP,Zhao, RH,Brenda, H,Hemmingsen, SM,&Xiao, W.(2001).Isolation by phage display and characterization of a single-chain antibody specific for O-6-methyldeoxyguanosine.CHINESE SCIENCE BULLETIN,46(12),1024-1029. |
| MLA | Dai, HP,et al."Isolation by phage display and characterization of a single-chain antibody specific for O-6-methyldeoxyguanosine".CHINESE SCIENCE BULLETIN 46.12(2001):1024-1029. |
入库方式： OAI收割
来源：水生生物研究所
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