Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion
文献类型：期刊论文
| 作者 | Hongtuo, F; Chingjiang, W |
| 刊名 | AQUACULTURE RESEARCH
 |
| 出版日期 | 2001-04-01 |
| 卷号 | 32期号:4页码:267-275 |
| 关键词 | electrofusion nuclear transfer UV inactivation fish oocyte blastomere gastrula cell |
| ISSN号 | 1355-557X |
| 通讯作者 | Hongtuo, F, Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Jiangsu, Peoples R China |
| 中文摘要 | To study nuclear transfer in the leach (Paramisgurnus dabryanus Sauvage), blastula and gastrula cells were fused with UV-inactivated oocytes by cell-to-cell electrofusion. To facilitate nuclear transfer, blastula and gastrula cells were cultured or incubated at 4 degreesC in different solutions. TC-199 medium supplemented with 20% calf serum was the best culture solution, and effectively retained the totipotence of blastula or gastrula cells for up to 10 days, It was found that gastrula cells incubated at 4 degreesC had the same totipotence as blastula cells, The optimal UV dosage for inactivation of the oocyte chromatin was 180-240 mJ cm(-2). Electrofusion was carried out in a cone-shaped fusion chamber, which permitted the recipient oocyte and the donor blastula cell to contact one another. The electrofusion procedure resulted in a 10% success rate of normal-appearing fish. Genetic analysis indicated that the nuclear material originated from the donor cell (blastomere) and the oocyte pronucleus did not take part in development. |
| 英文摘要 | To study nuclear transfer in the leach (Paramisgurnus dabryanus Sauvage), blastula and gastrula cells were fused with UV-inactivated oocytes by cell-to-cell electrofusion. To facilitate nuclear transfer, blastula and gastrula cells were cultured or incubated at 4 degreesC in different solutions. TC-199 medium supplemented with 20% calf serum was the best culture solution, and effectively retained the totipotence of blastula or gastrula cells for up to 10 days, It was found that gastrula cells incubated at 4 degreesC had the same totipotence as blastula cells, The optimal UV dosage for inactivation of the oocyte chromatin was 180-240 mJ cm(-2). Electrofusion was carried out in a cone-shaped fusion chamber, which permitted the recipient oocyte and the donor blastula cell to contact one another. The electrofusion procedure resulted in a 10% success rate of normal-appearing fish. Genetic analysis indicated that the nuclear material originated from the donor cell (blastomere) and the oocyte pronucleus did not take part in development. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries |
| 类目[WOS] | Fisheries |
| 研究领域[WOS] | Fisheries |
| 关键词[WOS] | CRASSOSTREA-GIGAS ; PACIFIC OYSTER ; ELECTRIC-FIELD ; MOUSE EMBRYO ; TRANSPLANTATION ; INDUCTION ; FUSION ; SURVIVAL ; LINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000168005600003 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/10022]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hongtuo, F,Chingjiang, W. Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion[J]. AQUACULTURE RESEARCH,2001,32(4):267-275. |
| APA | Hongtuo, F,&Chingjiang, W.(2001).Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion.AQUACULTURE RESEARCH,32(4),267-275. |
| MLA | Hongtuo, F,et al."Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion".AQUACULTURE RESEARCH 32.4(2001):267-275. |
入库方式： OAI收割
来源：水生生物研究所
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